应用化工
應用化工
응용화공
APPLIED CHEMICAL INDUSTRY
2014年
7期
1250-1254
,共5页
周卫%詹帅军%何利钦%陶科%侯太平
週衛%詹帥軍%何利欽%陶科%侯太平
주위%첨수군%하리흠%도과%후태평
蓖麻毒蛋白%提取工艺%响应面
蓖痳毒蛋白%提取工藝%響應麵
비마독단백%제취공예%향응면
ricin%extraction%resPonse surface methodology
以脱壳蓖麻籽为原料，在单因素实验的基础上，采用响应面法优化蓖麻粗毒蛋白的提取工艺。选取液料比、PH、浸提时间和硫酸铵饱和度4因素3水平进行中心组合实验。结果表明，蓖麻粗毒蛋白的最佳提取工艺条件为：液料比4.5 mL/g，PH 6.9，浸提时间38 h，硫酸铵饱和度65%。优化后蛋白提取率从2.82%提高到3.69%， SDS-PAGE凝胶电泳显示32 kD和34 kD两条蛋白带。
以脫殼蓖痳籽為原料，在單因素實驗的基礎上，採用響應麵法優化蓖痳粗毒蛋白的提取工藝。選取液料比、PH、浸提時間和硫痠銨飽和度4因素3水平進行中心組閤實驗。結果錶明，蓖痳粗毒蛋白的最佳提取工藝條件為：液料比4.5 mL/g，PH 6.9，浸提時間38 h，硫痠銨飽和度65%。優化後蛋白提取率從2.82%提高到3.69%， SDS-PAGE凝膠電泳顯示32 kD和34 kD兩條蛋白帶。
이탈각비마자위원료，재단인소실험적기출상，채용향응면법우화비마조독단백적제취공예。선취액료비、PH、침제시간화류산안포화도4인소3수평진행중심조합실험。결과표명，비마조독단백적최가제취공예조건위：액료비4.5 mL/g，PH 6.9，침제시간38 h，류산안포화도65%。우화후단백제취솔종2.82%제고도3.69%， SDS-PAGE응효전영현시32 kD화34 kD량조단백대。
Based on the single factor exPeriments,the resPonse surface methodology was emPloyed to oPti-mize the extraction Process for ricin from caster beans. The ratio of solid-to-solvent,PH,extraction time and ammonium sulfate saturation were used to carry out a 4-variable,3-level central comPosite design. The result showed that the oPtimal conditions were ratio of solid-to-solvent 4. 5 mL/g,PH 6. 9,extraction time 38 h and ammonium sulfate saturation 65%. Protein extraction yield under the oPtimal conditions was im-Proved from 2 . 82% to 3 . 69%. SDS-PAGE analysis showed that raw ricin contained 32 kD and 34 kD Protein band.
