CAJ | 학술논문

目的：了解铜陵地区乙型肝炎病毒（HBV）基因型的分布，分析该地区不同基因型别与肝功能损害、病毒复制水平及血清标志物的关系，探讨不同基因型的临床意义。方法采用聚合酶链反应（PCR）-反向点杂交法（RDB）对铜陵地区228例 HBV 阳性感染者的血清分别进行 HBV 基因型分型、HBV 载量以及乙型肝炎血清标志物和肝功能等的检测，并结合其指标进行相关性分析。结果在228例患者的血清样本中，226例样本成功分型，其中 B 型54．4％（124/228）、C 型37．3％（85/228）、B/C 混合型7．5％（17/228），2例未检出，占0．09％。不同 HBV 基因型患者间年龄、e 抗原（HBeAg）、e 抗体（抗 HBe）、肝功能的差异无统计学意义（P ＞0．05）。C 型HBV 载量明显高于 B 型及 B/C 混合型（P ＜0．01）。结论铜陵地区 HBV 基因型的分布以 B 型为主，其次为 C型和少量 B/C 混合型；C 型感染者 HBV 载量显著高于 B 型，C 型感染者肝脏损害较 B 型重，提示 HBV 基因型可能影响患者体内 HBV 复制并可能引起不同临床类型的慢性肝病。
목적：료해동릉지구을형간염병독（HBV）기인형적분포，분석해지구불동기인형별여간공능손해、병독복제수평급혈청표지물적관계，탐토불동기인형적림상의의。방법채용취합매련반응（PCR）-반향점잡교법（RDB）대동릉지구228례 HBV 양성감염자적혈청분별진행 HBV 기인형분형、HBV 재량이급을형간염혈청표지물화간공능등적검측，병결합기지표진행상관성분석。결과재228례환자적혈청양본중，226례양본성공분형，기중 B 형54．4％（124/228）、C 형37．3％（85/228）、B/C 혼합형7．5％（17/228），2례미검출，점0．09％。불동 HBV 기인형환자간년령、e 항원（HBeAg）、e 항체（항 HBe）、간공능적차이무통계학의의（P ＞0．05）。C 형HBV 재량명현고우 B 형급 B/C 혼합형（P ＜0．01）。결론동릉지구 HBV 기인형적분포이 B 형위주，기차위 C형화소량 B/C 혼합형；C 형감염자 HBV 재량현저고우 B 형，C 형감염자간장손해교 B 형중，제시 HBV 기인형가능영향환자체내 HBV 복제병가능인기불동림상류형적만성간병。
Objective To understand the distribution of genotypes in Tongling area,to analyze the relationship of different genotypes with liver function damage,virus replication and serum markers,and to investigate the clinical significance of different genotypes.Methods Polymerase chain reaction (PCR)-reverse dot blot (RDB)was used to determine hepatitis B virus (HBV)genotypes,HBV load,serum markers of hepatitis B and liver function among 228 patients infected with HBV in Tongling area.The correlation analysis was performed with other parameters.Results In 228 serum samples,226 samples were successfully genotyped,including 54.4% B type (124 /228),37.3% C type (85 /228)and 7.5% B/C hybrid type (17 /228),and 2 cases (0.09%)were not detected.Differences between different HBV genotypes and age,e antigen (HBeAg),anti-e antigen antibody (anti-HBe)and liver function had no statistical significance (P >0.05).The C type HBV load was significantly higher than those of B type and B/C hubrid type (P <0.01).Conclusions The distribution of HBV genotypes in Tongling area is dominated by B type,followed by C type and a small amount of B/C hybrid type.C type infected patients′HBV load is significantly higher than that of B type.The liver damage of patients with C type is more serious than that of B type,which prompts that HBV genotypes may influence on in vivo HBV replication and may cause chronic liver disease of different clinical types.