CAJ | 학술논문

目的：探讨右美托咪定预处理对大鼠肢体缺血再灌注肺损伤时脂质过氧化反应的影响。方法30只Wister雄性大鼠随机分成3组：假手术组（C组），肢体缺血再灌注组（M组），右美托咪定组（P组）。大鼠肢体缺血再灌注后肺损伤模型建立后，P组大鼠于缺血前30 min经尾静脉给予25μg/kg体重右美托咪定，其余两组给予相同容量的生理盐水。各组于再灌注末行血气分析，观察肺组织病理学变化，测定肺组织湿/干重比（W/D），测定肺组织中丙二醛（MDA）的含量和超氧化物歧化酶（SOD）活性。结果与C组比较，M组和P组均出现肺损伤；PaO2和SOD活性降低；W/D及MDA含量升高（P＜0．05）；与M组比较，P组肺组织损伤明显减轻；PaO2和SOD活性升高， W/D及MDA含量降低（P＜0．05）。结论右美托咪定预处理可抑制肢体缺血再灌注肺损伤大鼠脂质过氧化反应而发挥肺保护作用。
목적：탐토우미탁미정예처리대대서지체결혈재관주폐손상시지질과양화반응적영향。방법30지Wister웅성대서수궤분성3조：가수술조（C조），지체결혈재관주조（M조），우미탁미정조（P조）。대서지체결혈재관주후폐손상모형건립후，P조대서우결혈전30 min경미정맥급여25μg/kg체중우미탁미정，기여량조급여상동용량적생리염수。각조우재관주말행혈기분석，관찰폐조직병이학변화，측정폐조직습/간중비（W/D），측정폐조직중병이철（MDA）적함량화초양화물기화매（SOD）활성。결과여C조비교，M조화P조균출현폐손상；PaO2화SOD활성강저；W/D급MDA함량승고（P＜0．05）；여M조비교，P조폐조직손상명현감경；PaO2화SOD활성승고， W/D급MDA함량강저（P＜0．05）。결론우미탁미정예처리가억제지체결혈재관주폐손상대서지질과양화반응이발휘폐보호작용。
Objective To investigate influence of pretreatment with dexmedetomidine on lipid peroxidation during limb ischemia-reperfusion lung injury. Methods This study collected 30 Wistar rats and randomly divided them into 3 groups (10/each group):sham operation (C group), limb IR model group (M group),dexmedetomidine pretreatment group (P group). An animal model of ischemia in hind limbs and the reperfusion lung injury was made,afterward,P group received iv dexmedetomidine 25 μg/kg slowly at 30 min before ischemia. In C and M group the animals received equal volumes of NS instead of dexmedetomidine. The all rats were sacrificed at the end of reperfusion and the arterial blood was taken for blood gas analysis. The lungs were immediately removed for pathological examination and determination of the lung Wet/dry weight ratio (W/D). The values of malondialdehyde(MDA) and superoxide dismutase(SOD) in lung tissues were detected by colorimetry. Results Compared with C group,the damage in lung appeared in M and P group. PaO2 and the activity of SOD was decreased;W/D and MDA content was increased (P<0.05);compared with M group,the damage in lung was significantly reduced in P group,PaO2 and the activity of SOD was increased;W/D and MDA content was decreased (P<0.05). Conclusion Pretreatment with dexmedetomidine can inhibit lipid peroxidation during limb ischemia-reperfusion lung injury,thus it play a protective role for lung.