CAJ | 학술논문

本试验利用PCR技术扩增了伪狂犬病病毒（PRV）Kaplan株的糖蛋白G（gG）基因，结果显示，扩增的片段长约为1314 bp。将gG基因克隆到原核表达载体pET-30a中，转入大肠杆菌中并经IPTG诱导表达，通过SDS-PAGE蛋白电泳和Western-Blot免疫印迹分析，证实表达了分子量大小约为54 ku的特异性gG蛋白，并能被特异性抗体所识别。本研究为深入阐明PRV gG基因结构与功能及诊断试剂的研发奠定了基础。
본시험이용PCR기술확증료위광견병병독（PRV）Kaplan주적당단백G（gG）기인，결과현시，확증적편단장약위1314 bp。장gG기인극륭도원핵표체재체pET-30a중，전입대장간균중병경IPTG유도표체，통과SDS-PAGE단백전영화Western-Blot면역인적분석，증실표체료분자량대소약위54 ku적특이성gG단백，병능피특이성항체소식별。본연구위심입천명PRV gG기인결구여공능급진단시제적연발전정료기출。
The glycoprotein G (gG) gene of pseudorabies virus Kaplan strain was amplified by PCR followed by sequence analysis. The results revealed that the gene fragment was 1314 base pairs (bp) in length. The full-length gG gene was cloned into the prokaryotic expression vector pET-30a(+) , and expressed in E.coli induced by IPTG. The molecular weight of the gG protein was approximately 54ku identified by SDS-PAGE and Western-Blot.The results are helpful for investigat-ing the structure and function of gG protein and preparation of diagnostic reagents for pseudora-bies.