CAJ | 학술논문

目的：观察牙龈卟啉单胞菌脂多糖（Pg－lps）对体外培养大鼠血管平滑肌细胞（vascular smooth muscle cells，VSMCs）增殖和碱性磷酸酶（ALP）活性的影响。方法：采用组织块贴壁法培养大鼠VSMCs，传代培养并鉴定。Pg－lps （1～10000 ng／mL）干预VSMCs 0．5～4 d，采用CCK－8法和碱性磷酸酶试剂盒测定其增殖和ALP活性。结果：1 d后（1－10000 ng／mL）Pg－lps均显著促进VSMCs增殖，并随时间的延长，促进作用越明显；而（1 ng／mL、1μg／mL）Pg－lps在0．5～4 d均显著促进VSMCs的ALP活性，随着时间的延长促进作用更明显，且4 d时1μg／mL较1 ng／mL对ALP活性促进作用更明显。结论：Pg－lps能促进VSMCs的异常增殖；还可能通过促进VSMCs的ALP活性而影响其钙化过程，进而提示Pg－lps对心血管疾病的发生发展可能有重要作用。
목적：관찰아간계람단포균지다당（Pg－lps）대체외배양대서혈관평활기세포（vascular smooth muscle cells，VSMCs）증식화감성린산매（ALP）활성적영향。방법：채용조직괴첩벽법배양대서VSMCs，전대배양병감정。Pg－lps （1～10000 ng／mL）간예VSMCs 0．5～4 d，채용CCK－8법화감성린산매시제합측정기증식화ALP활성。결과：1 d후（1－10000 ng／mL）Pg－lps균현저촉진VSMCs증식，병수시간적연장，촉진작용월명현；이（1 ng／mL、1μg／mL）Pg－lps재0．5～4 d균현저촉진VSMCs적ALP활성，수착시간적연장촉진작용경명현，차4 d시1μg／mL교1 ng／mL대ALP활성촉진작용경명현。결론：Pg－lps능촉진VSMCs적이상증식；환가능통과촉진VSMCs적ALP활성이영향기개화과정，진이제시Pg－lps대심혈관질병적발생발전가능유중요작용。
Objective:To investigate the effect of Porphyromonas gingivalis lipopolysaccharide(Pg-lps)on proliferation and ALPase activity of rat vascular smooth muscle cells (VSMCs)in vitro. Method:The primary culture of VSMCs were cul-tured in vitro with the tissue adherent method. The cells were purified by differential adherent and natural growth purification and immunocytochemistry staining were used to identify the immunological characteristics.The proliferation and ALPase ac-tivity of cultured VSMCs by using CCK-8 method and alkaline phosphatase activity test. Result:After 1 day(1~10000 ng/mL) Pg-lps all promoted proliferation of VSMCs significantly,and as the extension of time promoting effect was more appar-ent;while(1 ng/mL,1 μg/mL) Pg-lps significantly promoted the ALP ase activity of VSMCs in(0.5~4 days),and as the extension of time promoting effect was more apparent;1μg/mL Pg-lps promoted ALPase activity stronger than the effect of 1 ng/mL Pg-lps. Conclusion:Pg-lps can promote abnormal proliferation of VSMCs;it possibly affects calcification process of VSMCs by promoting its ALPase activity;thus that prompts Pg-lps may play an important role for the development of car-diovascular disease.