临床儿科杂志
臨床兒科雜誌
림상인과잡지
2014年
7期
682-685
,共4页
高雪%尚小领%乔海霞%张彦霞%张存晖%张玉妥
高雪%尚小領%喬海霞%張彥霞%張存暉%張玉妥
고설%상소령%교해하%장언하%장존휘%장옥타
流感嗜血杆菌%生物膜%最低生物膜清除浓度%抗生素
流感嗜血桿菌%生物膜%最低生物膜清除濃度%抗生素
류감기혈간균%생물막%최저생물막청제농도%항생소
Haemophilus inlfuenzae%bioiflm%minimal biofilm eradication concentration%antibiotic
目的:体外建立流感嗜血杆菌(Haemophilus inlfuenzae)生物膜(BF),观察细菌形成BF后对抗生素敏感性的变化。方法从腺样体肥大患儿的离体腺样体组织中分离30株流感嗜血杆菌,并以96微孔板培养,采用结晶紫染色和扫描电镜(SEM)鉴定BF形成;分别测定氨苄西林(AMP)、头孢曲松(CRO)、左氧沙星(LVFX)和阿奇霉素(AZM)4种抗菌药物浮游菌的最低抑菌浓度(MIC)、最低杀菌浓度(MBC)以及生物膜菌最低生物膜清除浓度(MBEC)。结果30株流感嗜血杆菌体外都有不同程度BF形成。当BF形成后,不同抗生素的MBEC较其MIC、MBC增高程度大小不一,差异有统计学意义(MBEC/MBC:H=91.54,MBEC/MIC:H=87.91;P均<0.001)。AMP的MBEC最高,可高达MBC和MIC的100多倍;CRO 的MBEC较MBC和MIC可增高数十倍,LVFX和AZM对BF的MBEC最接近于MBC和MIC。结论 BF形成后,流感嗜血杆菌耐药性增强;氟喹诺酮类LVFX和大环内酯类AZM对流感嗜血杆菌BF作用相对良好。
目的:體外建立流感嗜血桿菌(Haemophilus inlfuenzae)生物膜(BF),觀察細菌形成BF後對抗生素敏感性的變化。方法從腺樣體肥大患兒的離體腺樣體組織中分離30株流感嗜血桿菌,併以96微孔闆培養,採用結晶紫染色和掃描電鏡(SEM)鑒定BF形成;分彆測定氨芐西林(AMP)、頭孢麯鬆(CRO)、左氧沙星(LVFX)和阿奇黴素(AZM)4種抗菌藥物浮遊菌的最低抑菌濃度(MIC)、最低殺菌濃度(MBC)以及生物膜菌最低生物膜清除濃度(MBEC)。結果30株流感嗜血桿菌體外都有不同程度BF形成。噹BF形成後,不同抗生素的MBEC較其MIC、MBC增高程度大小不一,差異有統計學意義(MBEC/MBC:H=91.54,MBEC/MIC:H=87.91;P均<0.001)。AMP的MBEC最高,可高達MBC和MIC的100多倍;CRO 的MBEC較MBC和MIC可增高數十倍,LVFX和AZM對BF的MBEC最接近于MBC和MIC。結論 BF形成後,流感嗜血桿菌耐藥性增彊;氟喹諾酮類LVFX和大環內酯類AZM對流感嗜血桿菌BF作用相對良好。
목적:체외건립류감기혈간균(Haemophilus inlfuenzae)생물막(BF),관찰세균형성BF후대항생소민감성적변화。방법종선양체비대환인적리체선양체조직중분리30주류감기혈간균,병이96미공판배양,채용결정자염색화소묘전경(SEM)감정BF형성;분별측정안변서림(AMP)、두포곡송(CRO)、좌양사성(LVFX)화아기매소(AZM)4충항균약물부유균적최저억균농도(MIC)、최저살균농도(MBC)이급생물막균최저생물막청제농도(MBEC)。결과30주류감기혈간균체외도유불동정도BF형성。당BF형성후,불동항생소적MBEC교기MIC、MBC증고정도대소불일,차이유통계학의의(MBEC/MBC:H=91.54,MBEC/MIC:H=87.91;P균<0.001)。AMP적MBEC최고,가고체MBC화MIC적100다배;CRO 적MBEC교MBC화MIC가증고수십배,LVFX화AZM대BF적MBEC최접근우MBC화MIC。결론 BF형성후,류감기혈간균내약성증강;불규낙동류LVFX화대배내지류AZM대류감기혈간균BF작용상대량호。
Objective To establish a bioiflm (BF) models of Haemophilus inlfuenza in vitro, and to observe the changes of antibiotic susceptibility after the BF fromation. Methods Thirty strains Haemophilus inlfuenzae isolated from adenoids of children with adenoidal hypertrophy and cultured in a 96-well plate. The BF was identiifed by crystal violet staining and scanning electron microscopy (SEM). The minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) and the minimum bioiflm bacteria bioiflm clear concentration (MBEC) of ampicillin (AMP), ceftriaxone (CRO), levolfoxacin (LVFX) and azithromycin (AZM) were individually detected. Result All of 30 strains of Haemophilus inlfuenzae formed various BF. After BF is formed, the increase of MBEC for different antibiotics was inconsistent with the increase of MIC and MBC. The difference was statistically signiifcant (MBEC/MBC, H=91.54;MBEC/MIC, H=87.91;all P<0.001). The MBEC of AMP was the highest, up to 100 times than the MBC and MIC. The MBEC of CRO was dozens of times than the MBC and MIC. The MBEC of LVFX and AZM were most close to those of MBC and MIC. Conclusion After the formation of BF, resistance to antibiotics of Haemophilus inlfuenzae is enhaced. LVFX and AZM showed more favorable effect on Haemophilus infuenzae BF.