CAJ | 학술논문

目的：评价多位点聚合酶链反应(PCR)在儿童结核分枝杆菌复合群鉴定中的临床价值。方法收集经初步鉴定为结核病分支杆菌的临床分离株，分别经传统PNB/TCH鉴定，以及选择7基因位点16SrRNA、Rv0577、IS1561、Rv1510、Rv1970、Rv3877/8及Rv3120，及4基因位点ropB、RD1、RD8(present)、RD8(deleted)，采用聚合酶链反应(PCR)对其进行扩增和鉴定。结果共收集204株临床分离菌株，传统PNB/TCH鉴定结果为，结核分枝杆菌199株，牛结核分枝杆菌3株，非结核分枝杆菌2株。4位点PCR鉴定结果为，结核分枝杆菌196株，牛结核分枝杆菌2株，卡介苗3株，非结核分枝杆菌3株。7位点PCR鉴定结果为，结核分枝杆菌191株，牛结核分枝杆菌2株，卡介苗3株，非洲分枝杆菌Ⅰ型4株，山羊分枝杆菌和田鼠分枝杆菌各1株，非结核分枝杆菌2株。结论两种PCR方法均较传统方法简便快捷，且均可较快的鉴定结核分枝杆菌复合群更多的亚种。7位点能鉴定出除非洲分枝杆菌Ⅱ型以外的所有儿童结核分枝杆菌复合群的亚种。4位点在鉴定卡介苗菌株中更加迅速简便。
목적：평개다위점취합매련반응(PCR)재인동결핵분지간균복합군감정중적림상개치。방법수집경초보감정위결핵병분지간균적림상분리주，분별경전통PNB/TCH감정，이급선택7기인위점16SrRNA、Rv0577、IS1561、Rv1510、Rv1970、Rv3877/8급Rv3120，급4기인위점ropB、RD1、RD8(present)、RD8(deleted)，채용취합매련반응(PCR)대기진행확증화감정。결과공수집204주림상분리균주，전통PNB/TCH감정결과위，결핵분지간균199주，우결핵분지간균3주，비결핵분지간균2주。4위점PCR감정결과위，결핵분지간균196주，우결핵분지간균2주，잡개묘3주，비결핵분지간균3주。7위점PCR감정결과위，결핵분지간균191주，우결핵분지간균2주，잡개묘3주，비주분지간균Ⅰ형4주，산양분지간균화전서분지간균각1주，비결핵분지간균2주。결론량충PCR방법균교전통방법간편쾌첩，차균가교쾌적감정결핵분지간균복합군경다적아충。7위점능감정출제비주분지간균Ⅱ형이외적소유인동결핵분지간균복합군적아충。4위점재감정잡개묘균주중경가신속간편。
Objective To evaluate the clinical value of multi-locus polymerase chain reaction (PCR) for identifying Mycobacterium tuberculosis complex isolated in children. Methods The isolates were collected and were first determined by PNB/TCH medium. 7-point PCR sites including 16SrRNA, Rv0577, IS1561, Rv1510, Rv1970, Rv3877/8 and Rv3120, and 4-point PCR sites including ropB, RD1, RD8 (present), RD8 (deleted) were used to amplify them by PCR. Results Total of 204 isolates were collected, in which 199 were Mycobacterium tuberculosis, 3 were Mycobacterium bovis, and 2 were non-tuberculous mycobacteria by the PNB/TCH method. 4-point PCR analysis showed that 196 were Mycobacterium tuberculosis, 2 were Mycobacterium bovis, 3 were BCG species and 3 were non-tuberculous mycobacteria. 7-point PCR analysis showed that 191 were Mycobacterium tuberculosis, 2 were Mycobacterium bovis, 3 were BCG species, 4 were African Mycobacterium type I, 1 was Mycobacterium caprae, 1 was Mycobacterium microti and 2 were non-tuberculous mycobacteria. Conclusion Compared with the conventional method, the PCR identification in 4-point PCR method and 7-point PCR method could rapidly identify the BCG among the complex group in children tuberculosis. 7-point PCR method was able to identify all the subspecies of Mycobacterium, except Africa Mycobacterium. 4-point PCR method would be more rapid and easier in the identification of BCG strains.