CAJ | 학술논문

目的：探讨BACTEC MGIT 960液体培养法和CLSI M24-A微量肉汤稀释法快速药敏试验对非结核分枝杆菌的意义。方法分别采用BACTEC MGIT 960液体培养法联合微量肉汤稀释法的快速药敏试验与L-J固体培养法和绝对浓度药敏法对48株非结核分枝杆菌进行培养，并对2种方法的培养时间和药敏报告时间进行比较。结果48株非结核分枝杆菌在BACTEC MGIT 960培养仪的平均阳性报告时间（7.0±5.3）d，L-J培养基平均阳性报告时间（17.5±10.2）d，两者比较差异有统计学意义（ P﹤0.05）。微量肉汤稀释法结果阳性报告时间为7~14d，L-J固体药敏试验阳性报告时间为28d，2种药敏试验方法对相同种类药物的耐药率分析结果差异无统计学意义（ P﹥0.05），微量肉汤稀释法比L-J法可以检测的药物种类更多。结论 BACTEC MGIT 960液体培养法联合微量肉汤稀释法快速药敏试验比L-J固体培养法和绝对浓度药敏法在培养时间、药敏结果种类和报告时间上优势明显。
목적：탐토BACTEC MGIT 960액체배양법화CLSI M24-A미량육탕희석법쾌속약민시험대비결핵분지간균적의의。방법분별채용BACTEC MGIT 960액체배양법연합미량육탕희석법적쾌속약민시험여L-J고체배양법화절대농도약민법대48주비결핵분지간균진행배양，병대2충방법적배양시간화약민보고시간진행비교。결과48주비결핵분지간균재BACTEC MGIT 960배양의적평균양성보고시간（7.0±5.3）d，L-J배양기평균양성보고시간（17.5±10.2）d，량자비교차이유통계학의의（ P﹤0.05）。미량육탕희석법결과양성보고시간위7~14d，L-J고체약민시험양성보고시간위28d，2충약민시험방법대상동충류약물적내약솔분석결과차이무통계학의의（ P﹥0.05），미량육탕희석법비L-J법가이검측적약물충류경다。결론 BACTEC MGIT 960액체배양법연합미량육탕희석법쾌속약민시험비L-J고체배양법화절대농도약민법재배양시간、약민결과충류화보고시간상우세명현。
Objective To study the BACTEC MGIT 960 liquid culture method and the CLSI M24-A broth microdilution method for rapid susceptibility testing the significance of non-tuberculous mycobacteria. Methods The BACTEC MGIT 960 liquid culture broth microdilution method combined rapid susceptibility testing,and L-J solid culture method combined the absolute concentration susceptibility method were respectively used in 48 strains with nontuberculous mycobacteri,the culture time and sensitivity reporting period of two combination methods were compared. Results The average positive report time in 48 strains of nontuberculous mycobacteria culture with BACTEC MGIT 960 instrument was(7. 0 ± 5. 3)d,while that of L-J media was(17. 5 ± 10. 2)d,in comparison the results of two methods were significantly different( P ﹤0 . 05 ),the reported time of broth microdilution France results was 7 -14 d,the report time of L-J solid susceptibility test was 28d,the comparison showed significant difference,two kinds of susceptibility testing methods for the same type of drug resistance rate analysis showed no significant difference( P﹥0 . 05 ),broth microdilution method can detect more types of drugs than the L-J method. Conclusion BACTEC MGIT 960 liquid culture broth microdilution method combined rapid susceptibility testing has obvious advantages over L-J solid culture method and the absolute concentration susceptibility method in the incubation time and susceptibility results reporting time types.