CAJ | 학술논문

目的：观察胰岛素受体底物（ insulin receptor substrate，IRS ）在多囊卵巢综合征（ polycystic ovarian syndrome，PCOS）患者子宫内膜的表达及酪氨酸磷酸化的程度，探讨其与胰岛素抵抗（ insulin resistance，IR）的相关性。方法 PCOS组30例，根据PCOS诊断标准选择；对照组30例，选择同期就诊的与PCOS组年龄匹配，平素月经周期规则，经检测血清激素、空腹血糖和胰岛素均在正常范围的妇女。PCOS组分为胰岛素抵抗亚组（ IR组）17例及非胰岛素抵抗亚组（非IR组）13例。采用免疫组织化学方法检测子宫内膜IRS-1和IRS-2蛋白的表达及IRS-1和IRS-2酪氨酸磷酸化的程度。结果 PCOS组子宫内膜IRS-1、IRS-2的蛋白表达和酪氨酸磷酸化程度显著弱于对照组（P﹤0.05）。PCOS伴IR组子宫内膜IRS-1、IRS-2的蛋白表达和酪氨酸磷酸化程度明显弱于PCOS非IR组（P﹤0.05）。结论 IRS-1和IRS-2蛋白及酪氨酸磷酸化程度在PCOS患者子宫内膜表达下降，伴IR的PCOS患者表达下降更明显。推测PCOS患者子宫内膜IRS的表达异常和酪氨酸磷酸化程度下降与子宫内膜IR的发生有密切关系。
목적：관찰이도소수체저물（ insulin receptor substrate，IRS ）재다낭란소종합정（ polycystic ovarian syndrome，PCOS）환자자궁내막적표체급락안산린산화적정도，탐토기여이도소저항（ insulin resistance，IR）적상관성。방법 PCOS조30례，근거PCOS진단표준선택；대조조30례，선택동기취진적여PCOS조년령필배，평소월경주기규칙，경검측혈청격소、공복혈당화이도소균재정상범위적부녀。PCOS조분위이도소저항아조（ IR조）17례급비이도소저항아조（비IR조）13례。채용면역조직화학방법검측자궁내막IRS-1화IRS-2단백적표체급IRS-1화IRS-2락안산린산화적정도。결과 PCOS조자궁내막IRS-1、IRS-2적단백표체화락안산린산화정도현저약우대조조（P﹤0.05）。PCOS반IR조자궁내막IRS-1、IRS-2적단백표체화락안산린산화정도명현약우PCOS비IR조（P﹤0.05）。결론 IRS-1화IRS-2단백급락안산린산화정도재PCOS환자자궁내막표체하강，반IR적PCOS환자표체하강경명현。추측PCOS환자자궁내막IRS적표체이상화락안산린산화정도하강여자궁내막IR적발생유밀절관계。
Objective To research the expression of protein insulin receptor substrate( IRS ) and the level of tyrosine phosphorylation in the endometrium of the patients with polycystic ovary syndrome( PCOS)and investigate the correlations between the expressions and the resistance of insulin. Methods Thirty patients with PCOS served as experimental group according to the diagnostic criteria, and 30 females hospitalized synchronously with regular menstrual cycle,normal serum sex hormone, fasting plasma glucose and fasting insulin levels as control group. According to the homeostasis model assessment for insulin resistance,the PCOS group was divided into two groups,insulin resistance group ( group IR)17 cases and non insulin resistance group( group NIR )13 cases. The differences in the expression of IRS-1 and IRS-2 by immunohistochemistry among group IR,group NIR and control group, and the levels of tyrosine phosphorylation of IRS-1 and IRS-2 among the three groups were compared. Results The expressions of protein IRS-1 and IRS-2 and the levels of tyrosine phosphorylation of IRS-1 and IRS-2 in PCOS group were significantly weaker than those in control group ( P ﹤0 . 05 ). The expressions of protein IRS-1 and IRS-2 and the levels of tyrosine phosphorylation of IRS-1 and IRS-2 in the PCOS group with IR were obviously weaker than those in the PCOS group without IR( P﹤0 . 05 ). Conclusion It is obvious that the expressions of protein IRS-1 and IRS-2 declined and the degrees of tyrosine phosphorylation descended in the patients with PCOS. The abnormality is quite distinct in the patients of PCOS group with IR. It speculates that there is a close relationship between the abnormal expression of IRS,the descent of the degree of tyrosine phosphorylation and the genesis of IR in the endometrium of the patients with PCOS.