CAJ | 학술논문

目的：观察重组沙门菌 TPIN 对小鼠黑色素瘤治疗的作用。方法应用小鼠黑色素瘤 B16细胞给16只 C57纯系小鼠建立荷黑色素瘤动物模型；将模型组随机分为对照组(PBS 组)及治疗组(TPIN),每组各8只。治疗组应用重组减毒沙门菌 TPIN 菌灌胃,对照组 PBS 缓冲溶液灌胃,每周1次共4次,在此期间记录瘤体体积。最后1次灌胃1周后,荷瘤小鼠心脏采血后处死,检测 CD3+/ CD4+、CD3+/ CD8+的 T 淋巴细胞表型的变化,瘤组织γ-干扰素(IFN-γ)含量及各组织脏器 CMV 的表达水平。结果成功构建了荷瘤小鼠模型,与治疗组比较,对照组肿瘤体积差异有显著性(P <0.01)；治疗组血 CD3、CD3+/ CD8+、肿瘤组织中 IFN-γ表达量显著高于对照组(P <0.05,P <0.01),治疗组小鼠的肠、肾、肝、脾、胃、肿瘤组织中均可扩增出与 CMV 瘤体大小相符的片段而对照组未扩增出相应片段。结论口服 DNA 疫苗 TPIN 免疫荷黑色素瘤小鼠可激起小鼠体内有效的免疫反应并能有效抑制瘤体的生长。
목적：관찰중조사문균 TPIN 대소서흑색소류치료적작용。방법응용소서흑색소류 B16세포급16지 C57순계소서건립하흑색소류동물모형；장모형조수궤분위대조조(PBS 조)급치료조(TPIN),매조각8지。치료조응용중조감독사문균 TPIN 균관위,대조조 PBS 완충용액관위,매주1차공4차,재차기간기록류체체적。최후1차관위1주후,하류소서심장채혈후처사,검측 CD3+/ CD4+、CD3+/ CD8+적 T 림파세포표형적변화,류조직γ-간우소(IFN-γ)함량급각조직장기 CMV 적표체수평。결과성공구건료하류소서모형,여치료조비교,대조조종류체적차이유현저성(P <0.01)；치료조혈 CD3、CD3+/ CD8+、종류조직중 IFN-γ표체량현저고우대조조(P <0.05,P <0.01),치료조소서적장、신、간、비、위、종류조직중균가확증출여 CMV 류체대소상부적편단이대조조미확증출상응편단。결론구복 DNA 역묘 TPIN 면역하흑색소류소서가격기소서체내유효적면역반응병능유효억제류체적생장。
Objective To observe the effect of oral recombinant attenuated salmonella typhimurium TPIN on melanoma in mice. Methods Mouse models of melanoma were established in 16 only inbred mice C57 using cultured mouse melanoma B16 injection; the models were randomly divided into control group ( PBS group, n = 8) and TPIN treatment (TPIN group, n = 8). TPIN group received gavage with recombinant Salmonella TPIN once a week for 4 times, while the PBS group was given phosphate buffered saline (PBS) with the same method, and tumor volumes were recorded during treatment. After treatment, heart blood of tumor-bearing mice was collected and the mice were sacrificed 1 week after the last gavage. The changes of CD3 + / CD4 + , CD3 + / CD8 + and T-lymphocyte phenotype were measured, and interferon-γ (IFN-γ) contents of tumor-bearing tissues and CMV expression levels of organs were detected. Results Mouse models of melanoma were successfully established. The differences in tumor volumes in TPIN group were statisti-cally significant, compared with those in PBS group (P < 0. 01); levels of CD3 and CD3 + / CD8 + in blood, and IFN-γ expression in tumor tissue in TPIN group were significantly increased, compared with those in PBS group (P < 0. 05). In TPIN group, CMV fragment could be amplified from intestine, kidney, liver, spleen, stomach and tumor tissues, while the fragment was not in the control group. Conclusion Oral TPIN DNA Vaccine can stimulate the effective im-mune action in melanoma mice, which can inhibit tumor growth of tumor-bearing mice as well.