CAJ | 학술논문

目的：探讨人胚胎源性人绒毛促性腺激素（HCG）在第3天胚胎培养液中的表达及其与早期胚胎发育间的关系。方法收集IVF-ET患者体外第3天单个培养胚胎培养液，通过ELISA方法检测培养液中HCG浓度，比较其与胚胎数目、分级、碎片等传统评分间的关系。结果在60个病人的163份第3天胚胎培养液标本中，胎源性HCG的检测率是93.8%（153/163），表达浓度是0.85±0.43 mIU/ml。HCG浓度与胚胎卵裂球细胞数呈正相关。随着细胞数增加，培养液中HCG表达也提高（P<0.05）。随着胚胎形态评分级别的下降，培养液中HCG表达下降（P<0.05）。结论 ELISA方法能检测出第3天胚胎培养液中胎源性HCG的微量表达。胚胎培养液中胎源性HCG水平与传统胚胎形态评估有相关性，提示它有可能作为新的评估胚胎发育的无创性方法。
목적：탐토인배태원성인융모촉성선격소（HCG）재제3천배태배양액중적표체급기여조기배태발육간적관계。방법수집IVF-ET환자체외제3천단개배양배태배양액，통과ELISA방법검측배양액중HCG농도，비교기여배태수목、분급、쇄편등전통평분간적관계。결과재60개병인적163빈제3천배태배양액표본중，태원성HCG적검측솔시93.8%（153/163），표체농도시0.85±0.43 mIU/ml。HCG농도여배태란렬구세포수정정상관。수착세포수증가，배양액중HCG표체야제고（P<0.05）。수착배태형태평분급별적하강，배양액중HCG표체하강（P<0.05）。결론 ELISA방법능검측출제3천배태배양액중태원성HCG적미량표체。배태배양액중태원성HCG수평여전통배태형태평고유상관성，제시타유가능작위신적평고배태발육적무창성방법。
Objective To investigate the association of human chorionic gonadotropin (HCG) level on day 3 of embryo culture with embryo development. Methods Spent culture media were collected from individually cultured embryos on day 3 of in vitro fertilization and embryo transfer (IVF-ET) cycles. HCG concentration in the culture media was measured using an ELISA kit and its association with embryo development was assessed. Results In the 163 samples of embryo culture media from 60 patients, HCG was positive in 153 sample (93.8%) with a mean level of 0.85 ± 0.43 mIU/ml. The concentration of hCG in the culture media increased gradually as the number of blastomeres increased (F=2.273, P=0.03), and decreased as the morphological grade of the embryo was lowered (F=3.900, P=0.02). Conclusion ELISA is capable of detecting HCG levels in spent culture media of embryos on day 3 of in vitro culture. The concentration of HCG in spent culture media is positively correlated with the status of early embryo development and implantation rate and thus serves as a useful marker for embryo selection in IVF-ET procedure.