CAJ | 학술논문

目的：研究重组减毒沙门菌菌株 TPIN 对 HepG2细胞生物学性能的影响。方法 TPIN 转染 HepG2细胞36 h 时收集细胞表达上清,MTT 法检测表达产物对 HepG2细胞生长的作用；Transwell 迁移实验观察 TPIN 表达产物对 HepG2细胞迁移的影响；将不同剂量表达产物分别加入甲基纤维素小碟,并将小碟小心放入鸡胚绒毛尿囊膜,37.8℃继续孵化3 d,观察 IL-2/ NK4表达产物体外对鸡胚绒膜尿囊膜血管的效应。结果 TPIN 表达上清显著抑制HepG2细胞增殖的活性,且有一定剂量依赖效应；表达产物可明显抑制肿瘤细胞迁移,且明显抑制鸡胚绒膜尿囊膜血管形成。结论 TPIN 在体外能高效转染肿瘤细胞,表达目的蛋白 IL-2及 NK4,且表达产物能够抑制 HepG2细胞的增殖和迁移,对鸡胚绒膜尿囊膜血管的形成有抑制效应。
목적：연구중조감독사문균균주 TPIN 대 HepG2세포생물학성능적영향。방법 TPIN 전염 HepG2세포36 h 시수집세포표체상청,MTT 법검측표체산물대 HepG2세포생장적작용；Transwell 천이실험관찰 TPIN 표체산물대 HepG2세포천이적영향；장불동제량표체산물분별가입갑기섬유소소설,병장소설소심방입계배융모뇨낭막,37.8℃계속부화3 d,관찰 IL-2/ NK4표체산물체외대계배융막뇨낭막혈관적효응。결과 TPIN 표체상청현저억제HepG2세포증식적활성,차유일정제량의뢰효응；표체산물가명현억제종류세포천이,차명현억제계배융막뇨낭막혈관형성。결론 TPIN 재체외능고효전염종류세포,표체목적단백 IL-2급 NK4,차표체산물능구억제 HepG2세포적증식화천이,대계배융막뇨낭막혈관적형성유억제효응。
Objective To study the effect of an recombinant of attenuated Salmonella typhimurium strain (TPIN) on cytobiology characteristics of HepG2 hepatoma carcinoma cells. Methods Cultured supernatant was collect-ed from TPIN-transfected HepG2 cells for 36 h, and the effect of expression products on HepG2 cell growth was detected by MTT assay; the effect of TPIN expression products on HepG2 cell migration was assessed by Transwell method; differ-ent dosages of expression products were put on the Methylcellulose small plates, and then the small plates were put into chick chorioallantoic membrane and was hatched at 37. 8℃ for 3 d, and then angiogenic effect of different dosages of IL-2 / NK4 expression products on chick embryo was observed. Results Supernatant collected from TPIN-transfected cells significantly inhibited the activity of HepG2 cell multiplioation with a certain dose-dependent effect; expression products significantly inhibited the cell migration as well as the angiogenesis in chick chorioallantoic membrane. Conclusion TPIN can effectively transfect HepG2 cells in vitro and express IL2 and NK4 stably, and expression products can inhibit HepG2 proliferation and migration as well as the angiogenesis inhibition of chick chorioallantoic membrane.