CAJ | 학술논문

目的：探讨不同剂量丙泊酚对大鼠MADB106细胞肺转移及肿瘤组织中MTA1和Wnt1表达的影响。方法 Fischer344雄性大鼠40只，随机分为：生理盐水组（S组）；脂肪乳剂组（F组）；丙泊酚30 mg/kg组（P30组）和50 mg/kg组（P50组），每组10只。1%戊巴比妥钠50 mg/kg腹腔注射经股静脉分别泵入等容量上述药物，1 h后均静注0.5 ml MADB106肿瘤细胞（2×105个）。3周后处死，计数肺转移瘤数目；免疫组化检测肺肿瘤组织中MTA1和Wnt1的表达，IPP软件定量分析。结果 F组和S组肺转移瘤数目及肿瘤组织中MTA1和Wnt1的表达无显著差异（P>0.05）。与S组相比，P30和P50组的肺转移瘤数目及肿瘤组织中MTA1和Wnt1的表达均显著减少（P<0.01），肺转移瘤数目及MTA1和Wnt1的表达与丙泊酚的剂量负相关，Pearson相关系数分别为-0.879、-0.980和-0.916（P<0.01）。MTA1和Wnt1的表达呈正相关，Pearson相关系数为0.902（P<0.01）。结论丙泊酚呈剂量依赖性抑制MADB106肿瘤细胞肺转移，并下调转移瘤中MTA1和Wnt1的表达。
목적：탐토불동제량병박분대대서MADB106세포폐전이급종류조직중MTA1화Wnt1표체적영향。방법 Fischer344웅성대서40지，수궤분위：생리염수조（S조）；지방유제조（F조）；병박분30 mg/kg조（P30조）화50 mg/kg조（P50조），매조10지。1%무파비타납50 mg/kg복강주사경고정맥분별빙입등용량상술약물，1 h후균정주0.5 ml MADB106종류세포（2×105개）。3주후처사，계수폐전이류수목；면역조화검측폐종류조직중MTA1화Wnt1적표체，IPP연건정량분석。결과 F조화S조폐전이류수목급종류조직중MTA1화Wnt1적표체무현저차이（P>0.05）。여S조상비，P30화P50조적폐전이류수목급종류조직중MTA1화Wnt1적표체균현저감소（P<0.01），폐전이류수목급MTA1화Wnt1적표체여병박분적제량부상관，Pearson상관계수분별위-0.879、-0.980화-0.916（P<0.01）。MTA1화Wnt1적표체정정상관，Pearson상관계수위0.902（P<0.01）。결론병박분정제량의뢰성억제MADB106종류세포폐전이，병하조전이류중MTA1화Wnt1적표체。
Objective To investigate the effects of different doses of propofol on pulmonary metastasis of intravenous injected tumor cells and expression of MTA1 and Wnt1 in the metastatic tumor in rats. Methods Forty male Fischer344 rats were randomly divided into 4 equal groups for intravenous administration of normal saline, intralipid, or propofol at the dose of 30 or 50 mg/kg pumped via the femoral vein. One hour after the infusion, MADB106 tumor cells (2 × 105) were injected intravenously in the rats. Pulmonary metastasis of the tumor cells was observed and the expression of MTA1 and Wnt1 in the metastatic tumor detected by immunohistochemistry 3 weeks later. Results The rats receiving saline and intralipid treatments showed a comparable number of pulmonary metastasis and similar expression levels of MTA1 and Wnt1 in the metastatic tumor (P>0.05); the tumor number and MTA1 and Wnt1 were significantly lower in the two propofol groups (P<0.01). The doses of propofol was inversely correlated with the number of pulmonary metastasis (r=-0.879) and expressions of MTA1 (r=-0.980) and Wnt1 (r=-0.916) (P<0.01), and MTA1 and Wnt1 expression levels in the metastatic tumors were closed correlated (r=0.902, P<0.01). Conclusion Propofol can dose-dependently suppress pulmonary metastasis of intravenously injected tumor cells and down-regulate MTA1 and Wnt1 expressions in the metastatic tumor tissue.