CAJ | 학술논문

目的：探讨替米沙坦对病毒性心肌炎小鼠脂联素，肿瘤坏死因子-α及BNP表达的影响。方法制备柯萨奇B3病毒感染的心肌炎小鼠60例，其中30例自病毒感染之日并连续7 d喂食替米沙坦作为观察组；另外30例无药物干预的心肌炎小鼠作为对照组；10例未感染病毒也无药物干预的小鼠作为空白组。观察3组小鼠的心肌病理组织学积分；双抗体夹心酶联免疫法测定血清脂联素、TNF-α、BNP浓度。结果观察组血清脂联素水平明显高于对照组，而 TNF-α、BNP、病理积分均低于对照组，差异有统计学意义（P＜0．05）。而且，与空白组相比，观察组血清脂联素、TNF-α、BNP、病理积分水平明显升高，差异有统计学意义（P＜0．05）。与空白组相比，对照组血清脂联素降低，而TNF-α、BNP、病理积分水平明显升高，差异有统计学意义（P＜0．05）。心肌病理积分与血清脂联素水平呈负相关性，与TNF-α，BNP水平具有正相关性。结论替米沙坦能够增加对心脏有保护作用的脂联素表达，减少TNF-α，BNP的分泌，减少心肌损伤。
목적：탐토체미사탄대병독성심기염소서지련소，종류배사인자-α급BNP표체적영향。방법제비가살기B3병독감염적심기염소서60례，기중30례자병독감염지일병련속7 d위식체미사탄작위관찰조；령외30례무약물간예적심기염소서작위대조조；10례미감염병독야무약물간예적소서작위공백조。관찰3조소서적심기병리조직학적분；쌍항체협심매련면역법측정혈청지련소、TNF-α、BNP농도。결과관찰조혈청지련소수평명현고우대조조，이 TNF-α、BNP、병리적분균저우대조조，차이유통계학의의（P＜0．05）。이차，여공백조상비，관찰조혈청지련소、TNF-α、BNP、병리적분수평명현승고，차이유통계학의의（P＜0．05）。여공백조상비，대조조혈청지련소강저，이TNF-α、BNP、병리적분수평명현승고，차이유통계학의의（P＜0．05）。심기병리적분여혈청지련소수평정부상관성，여TNF-α，BNP수평구유정상관성。결론체미사탄능구증가대심장유보호작용적지련소표체，감소TNF-α，BNP적분비，감소심기손상。
Objective To explore the role of Telmisartan induced expression of adiponectin ,TNF-αand BNP of viral myocarditis mice .Methods Preparation of coxsackie B3 virus myocarditis mice 60 cases of infection ,including 30 cases and feeding for 7 days from the date of virus infection telmisartan as observation group .Another 30 cases without drug intervention myocarditis mice as the control group .10 cases not mice infected with virus or drug intervention as blank group .The myocardial pathologic histology integral of three groups mice were observed .The concentrations of serum adi-ponectin ,TNF alpha ,BNP were determined by double antibody sandwich enzyme-linked immune method .Results The serum adiponectin levels in observation group was obviously higher than that of control group and TNF alpha ,BNP ,pathological integral were lower than the control group ,there were statistically significant differences (P< 0 .05) .Compared with the blank group ,observation group serum adiponectin ,TNF alpha ,BNP ,pathological integral level increased significantly ,there was a significant statistical difference (P<0 .05) .Compared with the blank group ,control group of serum adiponectin was reduced ,and TNF alpha ,BNP ,pathological integral level increased signifi-cantly ,there was a significant statistical difference (P<0 .05) .Myocardial pathological integral and serum adiponectin level was negatively correlation ,and TNF alpha ,BNP levels has a positive correla-tion .Conclusion Telmisartan can increase the expression on heart of adiponectin ,reduce TNF alpha , BNP secretion ,reduce myocardial injury.