CAJ | 학술논문

目的：以高效液相色谱法测定牛蒡子提取物中咖啡酰奎宁酸类化合物的含量。方法：色谱柱为Welch welchromC18（250mm×4.6mm，5μm），流动相为乙腈：0.1%磷酸（乙腈16%～18%0～60min梯度洗脱乙腈20%65min等度洗脱），流速为1.0ml·min-1，检测波长为327nm，柱温为室温。结果：3，5-二咖啡酰奎宁酸、1，5-二咖啡酰奎宁酸、3，4-二咖啡酰奎宁酸的进样量分别在4.28～12.84μg（r=0.9992）、4.16～12.48μg（r=0.9991）、4.20～12.60μg（r=0.9992）范围内与各自峰面积积分值呈良好线性关系；平均回收率分别为97.70%、98.50%、98.62%，RSD为1.80%、1.90%、1.20%。结论：本方法简便、快速、准确，可用于牛蒡子提取物中咖啡酰奎宁酸类化合物的质量控制。
목적：이고효액상색보법측정우방자제취물중가배선규저산류화합물적함량。방법：색보주위Welch welchromC18（250mm×4.6mm，5μm），류동상위을정：0.1%린산（을정16%～18%0～60min제도세탈을정20%65min등도세탈），류속위1.0ml·min-1，검측파장위327nm，주온위실온。결과：3，5-이가배선규저산、1，5-이가배선규저산、3，4-이가배선규저산적진양량분별재4.28～12.84μg（r=0.9992）、4.16～12.48μg（r=0.9991）、4.20～12.60μg（r=0.9992）범위내여각자봉면적적분치정량호선성관계；평균회수솔분별위97.70%、98.50%、98.62%，RSD위1.80%、1.90%、1.20%。결론：본방법간편、쾌속、준학，가용우우방자제취물중가배선규저산류화합물적질량공제。
Objective:To determine the contents of Caffeoylquinic Acids Compounds from the extractive of Fructus Arctii by HPLC. Methods:The determination was performed on Welch welchromC18 (250mm ×4.6mm,5μm)column with mobile phase consisted of acetonitrile:0.1% phosphoric acid [acetonitrile16%~18% 0~60 min gradient elution, acetonitrile20% 65 min isocratic elution],at flow rate of 1.0ml·min-1.The detection wavelength was set at 327 nm and the column temperature was room temperature. Results:The linear range of 3,5-dicaffeoylquinic acid was 4.28~12.84μg (r=0.9992)with an average recovery of 97.70%(RSD=1.80%,n=6). The linear range of 1,5-dicaffeoylquinic acid was 4.16~12.48μg (r=0.9991)with an average recovery of 98.50%(RSD=1.90%,n=6). The linear range of 3,4-dicaffeoylquinic acid was 4.20~12.60μg(r=0.9992)with an average recovery of 98.62%(RSD=1.20%,n=6).Conclusion:The method is simple, rapid and accurate for the quality control of Caffeoylquinic Acids Compounds in the extractive of Fructus Arctii.