北方药学
北方藥學
북방약학
JOURNAL OF NORTH PHARMACY
2014年
8期
11-11,12
,共2页
HPLC%巴洛沙星片%含量
HPLC%巴洛沙星片%含量
HPLC%파락사성편%함량
HPLC%Balofloxacin tablets%Determination
目的：建立RP-HPLC法[1]测定巴洛沙星的含量。方法：采用Ecosil C18色谱柱（250mm×4.6mm，5μm），0.05mol/L磷酸二氢钾溶液（含0.2%三乙胺，用磷酸调节pH值为3.0）∶乙腈（78∶22），流速为1ml·min-1，检测波长294nm。结果：采用外标法测定，方法的专属性强，巴洛沙星在48.7～195.0μg·ml-1范围内呈线性，线性方程为A=52.3268C-5.4290，相关系数为：r=0.9997（n=5）。平均回收率为99.1%（n=9；RSD=0.2%）。结论：该方法准确可靠，重现性好，可用于巴洛沙星片含量的测定。
目的：建立RP-HPLC法[1]測定巴洛沙星的含量。方法：採用Ecosil C18色譜柱（250mm×4.6mm，5μm），0.05mol/L燐痠二氫鉀溶液（含0.2%三乙胺，用燐痠調節pH值為3.0）∶乙腈（78∶22），流速為1ml·min-1，檢測波長294nm。結果：採用外標法測定，方法的專屬性彊，巴洛沙星在48.7～195.0μg·ml-1範圍內呈線性，線性方程為A=52.3268C-5.4290，相關繫數為：r=0.9997（n=5）。平均迴收率為99.1%（n=9；RSD=0.2%）。結論：該方法準確可靠，重現性好，可用于巴洛沙星片含量的測定。
목적：건립RP-HPLC법[1]측정파락사성적함량。방법：채용Ecosil C18색보주（250mm×4.6mm，5μm），0.05mol/L린산이경갑용액（함0.2%삼을알，용린산조절pH치위3.0）∶을정（78∶22），류속위1ml·min-1，검측파장294nm。결과：채용외표법측정，방법적전속성강，파락사성재48.7～195.0μg·ml-1범위내정선성，선성방정위A=52.3268C-5.4290，상관계수위：r=0.9997（n=5）。평균회수솔위99.1%（n=9；RSD=0.2%）。결론：해방법준학가고，중현성호，가용우파락사성편함량적측정。
Objective:To establish RP-HPLC method for the determination of Balofloxacin Content in Its Tablets. Method: The test used a Ecosil C18 column. The mobile phase consisted of 0.05mol/L potassium dihydrogen phosphate solution (containing 0.2% three triethylamine, pH value is adjusted to 3 with phosphoric acid)∶acetonitrile (78∶22). The flow rate was 1ml·min-1, detection wavelength was at 294nm. Result : Main component self-compare can be used when testing related substances. The method was sensitiveness and specificity.Balofloxacin had a good linear relation in the range of 48.7~195.0μg·ml-1, the calibration curve was A=52.3268C-5.4290,r=0.9997 (n=5),The average recovery rate was 99.1%(n=9; RSD=0.2%). Conclusion: The method is accurate and reliable,sensitive and rapid and it can be used for the determination of determination of balofloxacin content in its tablets.
