CAJ | 학술논문

目的：观察百合提取物（Lily extracted substances，LES）、知母提取物（Zhimu extracted substances，ZES）、百合知母汤提取物（Lily-Zhimu extracted substances，L-ZES）对慢性不可预见性温和刺激（Chronic unpredictable mild stress，CUMS）配合孤养所致大鼠抑郁模型的影响。方法：60只雄性SD大鼠随机分为正常组、模型组、百合提取物组、知母提取物组、百合知母汤提取物组、阳性药氟西汀组。采用CUMS配合孤养诱发抑郁模型。连续灌胃给药6周，观察大鼠体重变化，运用旷场实验、糖水偏爱实验评定大鼠的行为学特征。以酶联免疫吸附法（ELISA）检测血清多巴胺（DA）、促肾上腺皮质激素（ACTH）的含量。结果：与正常组相比，模型组大鼠的体重增加缓慢、行为学特征下降明显，血清DA含量下降、ACTH含量升高，差异有统计学意义（P<0.01），说明CUMS配合孤养可致大鼠出现抑郁行为。与模型组比较，百合提取物组、知母提取物组及百合知母汤提取物组、阳性药氟西汀（FHP）组能显著对抗抑郁刺激，大鼠的体重增加、行为学特征改善，DA的含量提高ACTH的含量降低。结论：百合提取物、知母提取物、百合知母汤提取物有很好的抗抑郁作用，其机制可能与调节抑郁症模型大鼠体内神经递质紊乱及调节HPA轴的功能、抑制大鼠HPA轴亢进有关。
목적：관찰백합제취물（Lily extracted substances，LES）、지모제취물（Zhimu extracted substances，ZES）、백합지모탕제취물（Lily-Zhimu extracted substances，L-ZES）대만성불가예견성온화자격（Chronic unpredictable mild stress，CUMS）배합고양소치대서억욱모형적영향。방법：60지웅성SD대서수궤분위정상조、모형조、백합제취물조、지모제취물조、백합지모탕제취물조、양성약불서정조。채용CUMS배합고양유발억욱모형。련속관위급약6주，관찰대서체중변화，운용광장실험、당수편애실험평정대서적행위학특정。이매련면역흡부법（ELISA）검측혈청다파알（DA）、촉신상선피질격소（ACTH）적함량。결과：여정상조상비，모형조대서적체중증가완만、행위학특정하강명현，혈청DA함량하강、ACTH함량승고，차이유통계학의의（P<0.01），설명CUMS배합고양가치대서출현억욱행위。여모형조비교，백합제취물조、지모제취물조급백합지모탕제취물조、양성약불서정（FHP）조능현저대항억욱자격，대서적체중증가、행위학특정개선，DA적함량제고ACTH적함량강저。결론：백합제취물、지모제취물、백합지모탕제취물유흔호적항억욱작용，기궤제가능여조절억욱증모형대서체내신경체질문란급조절HPA축적공능、억제대서HPA축항진유관。
Objective:To observe the antidepressant effect of LES;ZES;L-ZES on the model of the depressive rats that are induced by the CUMS and separate feeding method. Methods 60 male SD rats were randomLy divided into normal group, model group, LES group, ZES group, L-ZES group, positive drug fluoxetine (FHP)group. Use CUMS and separate feeding induced model of the depressive rats, All the rats are continuously gavaged for 6 weeks , and their data of changing weight were taken.And the Open Field Test and the Preference for Sucrose Test were used to assessed behavioral characteristics of the rats. The content of serum DA and ACTH were detected by using ELISA.Results Compared with the normal group,the model group gained weight slower and their behavioral characteristics decreased significantly. The DA content in serum decreased and the ACTH increased .The difference was statistically significant (P<0.01).It indicates that CUMS and isolated raising can induce depression phenomenon in rats .When it is compared with model group,the LES group; the ZES group;the L-ZES group and the FHP group could antagonize depression stimulation .The rats gained weight and improved the behavioral characteristics,and the content of DA increased and the ACTH’s decreased. Conclusion LES, ZES, L-ZES have a positive effect on antidepressant, and the mechanism be related to adjustment in vivo neurotransmitter disorders and regulation HPA axis hyperactivity.