CAJ | 학술논문

目的：建立小鼠腹腔动-静脉瘘模型及中药复方SP（30 mg· kg-1，丹酚酸B∶丹皮酚＝3∶1）对其影响。方法4号针头刺穿下腔静脉-腹主动脉联合壁造瘘，2 w后进行病理形态学分析，另一组动物造模后给予相应药物，连续2 w，全自动生化分析仪测定血清中乳酸脱氢酶（LDH）以及肌酸激酶（CK）的含量，计算全心重／体重和左室重／体重比，对左心室进行HE染色。结果造模4 w后，模型组全心重／体重比、左室重／体重比及血清中LDH、CK活性增高（ P＜0．01），病理形态学检查显示该模型复制成功；复方SP组全心重／体重比、左室重／体重比及血清中LDH、CK活性降低（ P＜0．01～0．05）。结论小鼠腹腔动-静脉模型复制成功，中药复方SP对小鼠腹腔动-静脉瘘导致的心肌肥厚有改善作用。
목적：건립소서복강동-정맥루모형급중약복방SP（30 mg· kg-1，단분산B∶단피분＝3∶1）대기영향。방법4호침두자천하강정맥-복주동맥연합벽조루，2 w후진행병리형태학분석，령일조동물조모후급여상응약물，련속2 w，전자동생화분석의측정혈청중유산탈경매（LDH）이급기산격매（CK）적함량，계산전심중／체중화좌실중／체중비，대좌심실진행HE염색。결과조모4 w후，모형조전심중／체중비、좌실중／체중비급혈청중LDH、CK활성증고（ P＜0．01），병리형태학검사현시해모형복제성공；복방SP조전심중／체중비、좌실중／체중비급혈청중LDH、CK활성강저（ P＜0．01～0．05）。결론소서복강동-정맥모형복제성공，중약복방SP대소서복강동-정맥루도치적심기비후유개선작용。
Objective To establish a model of arteriovenous fistula in mice and to evaluate the treatment of the compound SP (30 mg · kg-1 ,salvianolic acid B∶paeonol=3∶1 ) .Methods We created the fistula by using the size 4 needle piercing the abdominal aorta and getting into the inferior vena cava .Pathological analysis was made 2 weeks later.The other group was given some other medicines for two weeks after the establishment of the model .Two weeks after treatment ,LDH and CK in serum were determined ,the ratios of heart weight to body weight and left ventricular weight to body weight were calculated ,HE staining of the left ventricle was taken for the pa-thology.Results In the model group,the ratios of heart weight to body weight and the left ventricular weight to body weight and the LDH,CK levels in serum were all higher than those in the control group (P<0.01).Pathological analysis indicated the successfully re-produced model .The ratios of heart weight to body weight ,left ventricular weight to body weight and the CK ,LDH levels in the serum of the compound SP group compared with the control group were obviously decreased (P<0.01~0.05).Conclusions The model of ar-teriovenous fistula in mice reproduced successfully ,and compound SP improved the myocardial hypertrophy caused by arteriovenous fis -tula in mice.