CAJ | 학술논문

目的：探讨从患者体内获得有毒力和生物活性的呼吸道合胞病毒（ RSV）的方法。方法用咽拭子从患者咽部采集标本，离心后通过 RT - PCR 方法鉴定 RSV，随后从 RSV 阳性标本中，以人喉癌上皮细胞（Hep -2细胞）进一步分离和培养 RSV，并观察细胞致病变效应，检测病毒滴度。结果从60例患者咽拭子标本中采集分离到6株 RSV，阳性率为10％，且成功培养、传代，镜下观察 RSV 感染的细胞致病变效应明显，病毒滴度为106.33/100μL。结论采用咽拭子、Hep -2细胞和 RT - PCR 方法能够成功采集、分离、初步鉴定并且培养获得有毒力和生物活性的 RSV。
목적：탐토종환자체내획득유독력화생물활성적호흡도합포병독（ RSV）적방법。방법용인식자종환자인부채집표본，리심후통과 RT - PCR 방법감정 RSV，수후종 RSV 양성표본중，이인후암상피세포（Hep -2세포）진일보분리화배양 RSV，병관찰세포치병변효응，검측병독적도。결과종60례환자인식자표본중채집분리도6주 RSV，양성솔위10％，차성공배양、전대，경하관찰 RSV 감염적세포치병변효응명현，병독적도위106.33/100μL。결론채용인식자、Hep -2세포화 RT - PCR 방법능구성공채집、분리、초보감정병차배양획득유독력화생물활성적 RSV。
Objective To obtain live respiratory syncytial virus(RSV)from infected patients. Methods RSV in pharyngeal swab specimens collected from patients,after centrifugation,were identified by RT - PCR. RSV in positive specimens were further isolated and cultured through Hep - 2 cells,and the virus titers detected. Results Six RSV strains isolated from 60 specimens,with the positive rate of 10% ,were successfully cultured and passaged. Viral titer was 106. 33/ 100 μL. Conclusion RSV is a common pathogen of acute respiratory infections of infants. Using swabs,Hep - 2 cells and RT - PCR methods,virulent RSVs are successfully collected,isolated,and preliminary identified and cultured.