CAJ | 학술논문

为探索中华枸杞(Lycium chinense Mill. var. chinense)叶片离体再生技术，通过设置不同消毒时间梯度，研究了0.1%升汞溶液对中华枸杞叶片的消毒效果；以MS为基本培养基，添加6-BA、2，4-D、IAA等激素，研究了不同激素浓度配比对中华枸杞叶片离体再生的影响。结果表明，用0.1%升汞溶液消毒叶片8 min，污染率最低，是中华枸杞叶片的最佳消毒时间。基本培养基中添加1.0 mg/L 6-BA+0.2 mg/L 2，4-D是诱导愈伤组织的最佳激素浓度配比；基本培养基中添加1.0 mg/L 6-BA +0.1 mg/L NAA是诱导愈伤组织分化的最佳激素浓度配比；基本培养基中添加0.2 mg/L IAA +0.6 mg/L NAA是诱导丛生芽生根的适宜激素浓度配比。利用组织培养技术可有效实现中华枸杞叶片离体再生和快速繁殖，为其工厂化育苗和优良品种的选育奠定基础。
위탐색중화구기(Lycium chinense Mill. var. chinense)협편리체재생기술，통과설치불동소독시간제도，연구료0.1%승홍용액대중화구기협편적소독효과；이MS위기본배양기，첨가6-BA、2，4-D、IAA등격소，연구료불동격소농도배비대중화구기협편리체재생적영향。결과표명，용0.1%승홍용액소독협편8 min，오염솔최저，시중화구기협편적최가소독시간。기본배양기중첨가1.0 mg/L 6-BA+0.2 mg/L 2，4-D시유도유상조직적최가격소농도배비；기본배양기중첨가1.0 mg/L 6-BA +0.1 mg/L NAA시유도유상조직분화적최가격소농도배비；기본배양기중첨가0.2 mg/L IAA +0.6 mg/L NAA시유도총생아생근적괄의격소농도배비。이용조직배양기술가유효실현중화구기협편리체재생화쾌속번식，위기공엄화육묘화우량품충적선육전정기출。
To explore the vitro regeneration from leaves of Lycium chinense Mill. var. chinense, a gradient of different disin-fection time have been set up to study the effect of the 0.1%mercuric chloride solution to disinfect the leaves of L. chinense Mill. var. chinense. Depending on the MS base medium, the plant hormones 6-BA,2,4-D and IAA were added in the MS. Through the orthogonal experiment, the effects of different plant hormones concentration ratio on the vitro regeneration from leaves of L. chinense Mill. var. chinense have been studied. The pollution rate was the lowest when the leaves were dealt with 0.1% mercuric chloride solution in 8 min (the best sterilization time). MS medium with 1.0 mg/L 6-BA and 0.2 mg/L 2,4-D was the best hormone concentration ratio to induce callus. MS medium with 1.0 mg/L 6-BA and 0.1 mg/L NAA was the best hormone concentration ratio to induce callus differentiation. Using tissue culture technology can not only effectively achieve the vitro regeneration and rapid propagation from leaves of L. chinense Mill. var. chinense, but also lay foundation for industrialized seedling production and the seed selection.