CAJ | 학술논문

采用超声波辅助萃取法提取，高效液相色谱法测定了锁阳(Cynomorium songaricum)中的熊果酸含量。在单因素试验基础上，以乙醇体积分数、料液比、超声时间、超声功率为研究对象，进行了4因素3水平正交试验。结果表明，熊果酸在4.0~16.0μg/μL范围内呈良好线性关系，回归方程为y=622.7x-85.517，r=0.9998，加样回收率为98.08%。锁阳中熊果酸最佳提取工艺为：超声功率150 W，料液比1：25，85%(V/V ，下同)乙醇超声提取30 min，在此条件下，锁阳中熊果酸含量为1.53%。该工艺能有效提取锁阳中熊果酸，为评价中药材中的熊果酸水平提供参考。
채용초성파보조췌취법제취，고효액상색보법측정료쇄양(Cynomorium songaricum)중적웅과산함량。재단인소시험기출상，이을순체적분수、료액비、초성시간、초성공솔위연구대상，진행료4인소3수평정교시험。결과표명，웅과산재4.0~16.0μg/μL범위내정량호선성관계，회귀방정위y=622.7x-85.517，r=0.9998，가양회수솔위98.08%。쇄양중웅과산최가제취공예위：초성공솔150 W，료액비1：25，85%(V/V ，하동)을순초성제취30 min，재차조건하，쇄양중웅과산함량위1.53%。해공예능유효제취쇄양중웅과산，위평개중약재중적웅과산수평제공삼고。
Ursolic acid in Cynomorium songaricum was ultrasonically extracted and detected by HPLC. According to singlefactor experiments,three levels of four factors including ethanol concentration,material -liquid ratio,ultrasonic time, and ultrasonic power were selected for the orthogonal design.The results showed that the calibration curve of ursolic acid was linear (r=0.999 8) in the range of 4.0~16.0 μg/μL.The equation was y=622.7x-85.517,r=0.999 8,and recovery was 98.08%. The optimum extraction was carried out in 85%(V / V) ethanol for 30 min with the ultrasonic power of 150 W,and the material-liquid ratio 1:25.Under the optimal conditions,the content of ursolic acid was 1.53%.The method can extract ursolic acid efficiently,and provide a reference for evaluation ursolic acid in medicinal materials.