CAJ | 학술논문

采用超声提取和RP-HPLC法分析了6种冬青属苦丁茶新干燥的功能叶及苦丁茶冬青植物在不同发育阶段、不同地域、野生与栽培材料、不同存放时间下功能叶中的绿原酸含量。结果表明，不同样品材料中的绿原酸含量由大到小排序依次为苦丁茶冬青、霍山冬青、大叶冬青、五棱苦丁茶、华中枸骨、枸骨；新干燥的成熟叶样品在室温下存放2个月后绿原酸的含量显著下降；在野生的苦丁茶冬青叶片不同发育阶段中，一般是以老叶样品中的绿原酸含量高；野生苦丁茶冬青植株叶片中绿原酸的含量要高于栽培植株叶片中绿原酸的含量。6种冬青属苦丁茶叶片样品中的绿原酸含量存在较大的种间差异。
채용초성제취화RP-HPLC법분석료6충동청속고정다신간조적공능협급고정다동청식물재불동발육계단、불동지역、야생여재배재료、불동존방시간하공능협중적록원산함량。결과표명，불동양품재료중적록원산함량유대도소배서의차위고정다동청、곽산동청、대협동청、오릉고정다、화중구골、구골；신간조적성숙협양품재실온하존방2개월후록원산적함량현저하강；재야생적고정다동청협편불동발육계단중，일반시이로협양품중적록원산함량고；야생고정다동청식주협편중록원산적함량요고우재배식주협편중록원산적함량。6충동청속고정다협편양품중적록원산함량존재교대적충간차이。
Chlorogenic acid in the mature leaf was extracted ultrasonically and analyzed by RP-HPLC from six Ilex species and I.kudingcha in different developing stage,region and storaging time,wild and cultivated samples. The results showed that the content of chlorogenic acid in fresh mature leaves of six species was I. kudingcha> I. huoshanesis> I. latifolia > I. centrochinensis > I. pentagona > I. cornuta. The significant decrease of chlorogenic acid in dried fresh leaf was observed after two months. In general,its content in old leaf was highest among different developing stage,and the content of wild leaf was higher than cultivation one. There were large differences among six Ilex species in the contents of chlorogenic acid.