CAJ | 학술논문

试验运用原位聚合法，在引发剂诱发作用下聚合了疏水性单体甲基丙烯酸苄基酯(BMA)、带负电的2-丙烯酸酰胺2-甲基丙磺酸(AMPS)和交联能力强的交联剂三羟甲基丙烷三甲基丙烯酸酯(TMPT-MA)。通过优化致孔剂比例和环己醇比例，结合电镜图观察，当致孔剂含量为70%时，整体柱不仅通透性好，而且不容易坍塌、剥落。当致孔剂中环己醇比例为55%时，整体柱背压最低，通透性最好，同时用毛细管电色谱(CEC)对该整体柱的表征为柱大、中、小孔都具备，且空隙均匀，柱效高，渗透性好，重现性和柱寿命良好。
시험운용원위취합법，재인발제유발작용하취합료소수성단체갑기병희산변기지(BMA)、대부전적2-병희산선알2-갑기병광산(AMPS)화교련능력강적교련제삼간갑기병완삼갑기병희산지(TMPT-MA)。통과우화치공제비례화배기순비례，결합전경도관찰，당치공제함량위70%시，정체주불부통투성호，이차불용역담탑、박락。당치공제중배기순비례위55%시，정체주배압최저，통투성최호，동시용모세관전색보(CEC)대해정체주적표정위주대、중、소공도구비，차공극균균，주효고，삼투성호，중현성화주수명량호。
A capillary electrochromatography monolithic column with hydrophobic stationary phases was prepared in situ poly-merization of benzyl methacrylate, trimethylolpropane three methyl acrylate and 2-acrylic acid amides- 2-methyl propyl sul-fonic acid in the presence of porogens. Through optimizing the ratio of porogens and the ratio of cyclohexanol in porogens, the column achieve good permeability with diverse holes when the percentage was 70%. The backpressure of monolithic col-umn reached the lowest and the permeability was the best when the percentage of cyclohexanol was 55%. Based on this col-umn, repeatability, column life, electroosmotic flow and efficiency were characterized under CEC mode. The interspace was even, column efficiency high, the permeability good while the reproducibility was good and the column life was long.