中华临床医师杂志（电子版）
中華臨床醫師雜誌（電子版）
중화림상의사잡지（전자판）
CHINESE JOURNAL OF CLINICIANS(ELECTRONIC VERSION)
2014年
13期
2474-2479
,共6页
李平%郭小波%刘宇强%刘强%范晓洁
李平%郭小波%劉宇彊%劉彊%範曉潔
리평%곽소파%류우강%류강%범효길
股骨头坏死%血管内皮生长因子类%骨形态发生蛋白质类%体外冲击波疗法
股骨頭壞死%血管內皮生長因子類%骨形態髮生蛋白質類%體外遲擊波療法
고골두배사%혈관내피생장인자류%골형태발생단백질류%체외충격파요법
Femur head necrosis%Vascular endothelial growth factors%Bone morphogenetic proteins%Extracorporeal shock wave therapy
目的：观察体外冲击波疗法对激素性股骨头缺血坏死中血管内皮生长因子（VEGF）和骨形态发生蛋白（BMP）的影响。方法新西兰大白兔40只，Yamamoto法制造模成功后，左侧股骨头做冲击波治疗设为实验组（A组），右侧股骨头不做冲击波治疗设为对照组（B组）。A组分别于治疗后1、2、4、8、12周处死动物，每次随机处死8只，取双侧股骨头行大体标本HE染色及细胞计数，免疫组化观察VEGF和BMP的表达。结果 HE染色观察在体外冲击波冲击后4、8、12周，毛细血管计数平均值分别为9.95±2.14，14.70±3.08，13.15±3.07，而B组分别为4.85±1.67，4.30±1.63，4.40±1.79；成骨细胞计数A组平均值为33.35±3.39，47.35±5.20，53.40±5.09，而B组分别为24.1±2.45，21.70±4.08，19.60±4.28，HE染色两组比较差异均有统计学意义（P＜0.05）。免疫组化染色在体外冲击波冲击后的2、4、8、12周VEGF表达A组平均值为0.104±0.018，0.177±0.020，0.167±0.017，0.144±0.031，而B组分别为0.074±0.017，0.078±0.021，0.078±0.023，0.070±0.019；且BMP表达A组平均值为0.210±0.028，0.304±0.039，0.292±0.052，0.268±0.053，而B组分别为0.136±0.025，0.140±0.033，0.144±0.029，0.118±0.028，免疫组化染色两组比较差异均有统计学意义（P＜0.05）。结论体外冲击波疗法可促进兔激素性股骨头缺血坏死中VEGW和BMP的表达。
目的：觀察體外遲擊波療法對激素性股骨頭缺血壞死中血管內皮生長因子（VEGF）和骨形態髮生蛋白（BMP）的影響。方法新西蘭大白兔40隻，Yamamoto法製造模成功後，左側股骨頭做遲擊波治療設為實驗組（A組），右側股骨頭不做遲擊波治療設為對照組（B組）。A組分彆于治療後1、2、4、8、12週處死動物，每次隨機處死8隻，取雙側股骨頭行大體標本HE染色及細胞計數，免疫組化觀察VEGF和BMP的錶達。結果 HE染色觀察在體外遲擊波遲擊後4、8、12週，毛細血管計數平均值分彆為9.95±2.14，14.70±3.08，13.15±3.07，而B組分彆為4.85±1.67，4.30±1.63，4.40±1.79；成骨細胞計數A組平均值為33.35±3.39，47.35±5.20，53.40±5.09，而B組分彆為24.1±2.45，21.70±4.08，19.60±4.28，HE染色兩組比較差異均有統計學意義（P＜0.05）。免疫組化染色在體外遲擊波遲擊後的2、4、8、12週VEGF錶達A組平均值為0.104±0.018，0.177±0.020，0.167±0.017，0.144±0.031，而B組分彆為0.074±0.017，0.078±0.021，0.078±0.023，0.070±0.019；且BMP錶達A組平均值為0.210±0.028，0.304±0.039，0.292±0.052，0.268±0.053，而B組分彆為0.136±0.025，0.140±0.033，0.144±0.029，0.118±0.028，免疫組化染色兩組比較差異均有統計學意義（P＜0.05）。結論體外遲擊波療法可促進兔激素性股骨頭缺血壞死中VEGW和BMP的錶達。
목적：관찰체외충격파요법대격소성고골두결혈배사중혈관내피생장인자（VEGF）화골형태발생단백（BMP）적영향。방법신서란대백토40지，Yamamoto법제조모성공후，좌측고골두주충격파치료설위실험조（A조），우측고골두불주충격파치료설위대조조（B조）。A조분별우치료후1、2、4、8、12주처사동물，매차수궤처사8지，취쌍측고골두행대체표본HE염색급세포계수，면역조화관찰VEGF화BMP적표체。결과 HE염색관찰재체외충격파충격후4、8、12주，모세혈관계수평균치분별위9.95±2.14，14.70±3.08，13.15±3.07，이B조분별위4.85±1.67，4.30±1.63，4.40±1.79；성골세포계수A조평균치위33.35±3.39，47.35±5.20，53.40±5.09，이B조분별위24.1±2.45，21.70±4.08，19.60±4.28，HE염색량조비교차이균유통계학의의（P＜0.05）。면역조화염색재체외충격파충격후적2、4、8、12주VEGF표체A조평균치위0.104±0.018，0.177±0.020，0.167±0.017，0.144±0.031，이B조분별위0.074±0.017，0.078±0.021，0.078±0.023，0.070±0.019；차BMP표체A조평균치위0.210±0.028，0.304±0.039，0.292±0.052，0.268±0.053，이B조분별위0.136±0.025，0.140±0.033，0.144±0.029，0.118±0.028，면역조화염색량조비교차이균유통계학의의（P＜0.05）。결론체외충격파요법가촉진토격소성고골두결혈배사중VEGW화BMP적표체。
Objective To observe the VEGF and BMP in the glucocorticoid-induced ischemic necrosis of femoral head treated with extracorporeal shock wave therapy.Methods40 New Zealand rabbits were modeled in Yamamoto. Experimental group (group A): the left side of the rabbits, femoral head treated with extracorporeal shock wave therapy; contrast group (group B): the right side of the rabbits, femoral head without any treatment. After 1, 2, 4, 8, 12 weeks random eight rabbits to be put to death at a time.Both sides of all the rabbits, femoral head were observed in HE staining for cell count, the expression of VEGF and BMP immunohistochemical observation.Results HE dyeing observation after 4, 8, 12 weeks, group A blood capillary count average were 9.95±2.14, 14.70±3.08, 13.15±3.07, and group B were 4.85±1.67, 4.30±1.63, 4.40±1.79; Osteoblast count group A mean value of 33.35±3.39, 47.35±5.20, 53.40±5.09, while group B were 24.1±2.45, 21.70±4.08, 19.60±4.28, HE staining was similar between the two groups had statistical significance (P<0.05). Immunohistochemical staining after 2, 4, 8, 12 weeks of VEGF expression in group A mean value of 0.104±0.018, 0.177±0.020, 0.167±0.017, 0.144±0.031, while group B were 0.074±0.017, 0.078±0.021, 0.078±0.023, 0.070±0.019; And the expression of BMP group A mean value of 0.210±0.028, 0.304±0.039, 0.292±0.052, 0.268±0.053, while group B were 0.136±0.025, 0.140±0.033, 0.144±0.029, 0.118±0.028, immunohistochemical staining was similar between the two groups had statistical significance (P<0.05).Conclusion Extracorporeal shock wave therapy can promote rabbit VEGF and BMP in the glucocorticoid-induced ischemic necrosis of femoral head.
