中国伤残医学
中國傷殘醫學
중국상잔의학
CHINESE JOURNAL OF TRAUMA AND DISABILITY MEDICINE
2014年
14期
11-13
,共3页
白登彦%张海军%袁治国
白登彥%張海軍%袁治國
백등언%장해군%원치국
Neurogenin2基因%真核表达质粒
Neurogenin2基因%真覈錶達質粒
Neurogenin2기인%진핵표체질립
Neurogenin2 gene%Eukaryotic expression plasmid
目的：构建大鼠Neurogenin2（Ngn2）基因的真核表达质粒。方法：从大鼠海马组织提取海马总mRNA ，RT-PCR获得Ngn2基因cDNA，构建重组质粒pSecTag2／HygroB-Ngn2， XhoⅠ和HindⅢ双酶切、测序鉴定重组质粒。结果：RT-PCR获得片断与预期结果相符，酶切鉴定、测序鉴定证实pSecTag2／HygroB-Ngn2重组质粒构建成功。结论：成功构建了大鼠Ngn2基因的真核表达质粒，并对该基因的真核表达产物进行鉴定，为下一步Ngn2基因对神经损伤修复作用的研究奠定基础。
目的：構建大鼠Neurogenin2（Ngn2）基因的真覈錶達質粒。方法：從大鼠海馬組織提取海馬總mRNA ，RT-PCR穫得Ngn2基因cDNA，構建重組質粒pSecTag2／HygroB-Ngn2， XhoⅠ和HindⅢ雙酶切、測序鑒定重組質粒。結果：RT-PCR穫得片斷與預期結果相符，酶切鑒定、測序鑒定證實pSecTag2／HygroB-Ngn2重組質粒構建成功。結論：成功構建瞭大鼠Ngn2基因的真覈錶達質粒，併對該基因的真覈錶達產物進行鑒定，為下一步Ngn2基因對神經損傷脩複作用的研究奠定基礎。
목적：구건대서Neurogenin2（Ngn2）기인적진핵표체질립。방법：종대서해마조직제취해마총mRNA ，RT-PCR획득Ngn2기인cDNA，구건중조질립pSecTag2／HygroB-Ngn2， XhoⅠ화HindⅢ쌍매절、측서감정중조질립。결과：RT-PCR획득편단여예기결과상부，매절감정、측서감정증실pSecTag2／HygroB-Ngn2중조질립구건성공。결론：성공구건료대서Ngn2기인적진핵표체질립，병대해기인적진핵표체산물진행감정，위하일보Ngn2기인대신경손상수복작용적연구전정기출。
Objective:To construct eukaryotic expression plasmid of rat Neurogenin 2 (Ngn2).Methods:The total RNA was extracted from the hippocampus of rats .The cDNA encoding Ngn 2 gene was amplified by RT-PCR, cloned and constructed the recombinant plas-mid pSecTag2/HygroB-Ngn2.The recombinant plasmid was identified by restriction enzyme Xho Ⅰand Hind III cutting and sequence a-nalysis .Results:The product of RT-PCR was coincide with what we preconceived .Restriction enzyme XhoⅠand Hind III cutting and se-quence analysis proved the recombinant plasmid to be pSecTag 2/HygroB-Ngn2.It was constructed successfully .Conclusion:The success-ful cloning of rat Ngn2 gene might lay a basis for studying the effects on its nerve repair.
