CAJ | 학술논문

目的：探讨津力达对胰岛素抵抗大鼠骨骼肌GLUT4表达的影响。方法 SD大鼠分为正常对照组（ ND组），高脂对照组（ HFD组），津力达组及吡格列酮组，每组6只；灌胃8周后，进行腹腔注射葡萄糖耐量试验并计算曲线下面积（ AUC）；测得大鼠空腹血糖、胰岛素计算HOMA-IR指数；RT-PCR及western-blotting检测大鼠骨骼肌GLUT4表达。结果津力达明显改善了30 min、60 min血糖及AUC，吡格列酮改善了60、120 min血糖及AUC（ P ＜0．05）；津力达及吡格列酮明显改善HOMA-IR指数（ P ＜0．05）；津力达及吡格列酮明显上调了骨骼肌GLUT4的表达。结论津力达能够上调大鼠骨骼肌GLUT4的表达，改善高脂饮食诱导的胰岛素抵抗。
목적：탐토진력체대이도소저항대서골격기GLUT4표체적영향。방법 SD대서분위정상대조조（ ND조），고지대조조（ HFD조），진력체조급필격렬동조，매조6지；관위8주후，진행복강주사포도당내량시험병계산곡선하면적（ AUC）；측득대서공복혈당、이도소계산HOMA-IR지수；RT-PCR급western-blotting검측대서골격기GLUT4표체。결과진력체명현개선료30 min、60 min혈당급AUC，필격렬동개선료60、120 min혈당급AUC（ P ＜0．05）；진력체급필격렬동명현개선HOMA-IR지수（ P ＜0．05）；진력체급필격렬동명현상조료골격기GLUT4적표체。결론진력체능구상조대서골격기GLUT4적표체，개선고지음식유도적이도소저항。
Objective To investigate the effect of Jinlida on the expression of GLUT 4 in skeletal muscle of rats with insulin resistance .Methods Sprague Dawley ( SD ) rats were randomly divided into four groups:normal diet group ( ND group),high-fat diet group (HFD group),high fat diet with Jinlida group (Jinlida group) and high fat diet with pioglitazone group (pioglitazone group),with 6 rats in each group.After 8 weeks,intraperitoneal glucose tolerance test (IPGTT) was performed and area under the curve ( AUC) was calculated based on the blood glucose curves;blood glucose and insulin were detected to calculate HOMA-IR;the expression levels of GLUT 4 in skeletal muscle were detected by quantitative real-time polymerase chain reaction (RT-PCR) and Western Blot.Results Jinlida administration obviously improved 30min,60min blood glucose and AUC during IPGTT,pioglitazone improved significantly 60min,120min blood glucose and AUC ( P <0.05),both Jinlida and pioglitazone improved obviously HOMA-IR index ( P <0.05),and both of them up-regulated the expression of GLUT4 in skeletal muscle ( P <0.05).Conclusion Jinlida can up-regulate expression of GLUT4 in skeletal muscle of rats,as a result,which can improve insulin resistance induced by high fat diet .