CAJ | 학술논문

目的： PD-1基因敲除后会激活T细胞并提高机体炎症水平并可能影响机体氧化应激水平，本研究旨在观察C57 BL/6小鼠在PD-1基因敲除后心房细胞氧化应激水平变化及其对小鼠心房结构重构的影响。方法我们同时对两组动物进行了观察：C57BL/6小鼠和C57BL/6-PD-1-/-小鼠。对比观察两组心房肌细胞氧化应激水平和心房肌心肌纤维化水平。结果和对照组相比较，C57 BL/6-PD-1-/-小鼠心房肌细胞细胞内活性氧水平明显升高，同时心房肌Masson染色显示基因敲除后心房肌出现了纤维化，而对照组没有出现。结论 PD-1基因敲除后出现心房肌细胞活性氧水平的升高并导致了C57 BL/6小鼠的心房结构重构。
목적： PD-1기인고제후회격활T세포병제고궤체염증수평병가능영향궤체양화응격수평，본연구지재관찰C57 BL/6소서재PD-1기인고제후심방세포양화응격수평변화급기대소서심방결구중구적영향。방법아문동시대량조동물진행료관찰：C57BL/6소서화C57BL/6-PD-1-/-소서。대비관찰량조심방기세포양화응격수평화심방기심기섬유화수평。결과화대조조상비교，C57 BL/6-PD-1-/-소서심방기세포세포내활성양수평명현승고，동시심방기Masson염색현시기인고제후심방기출현료섬유화，이대조조몰유출현。결론 PD-1기인고제후출현심방기세포활성양수평적승고병도치료C57 BL/6소서적심방결구중구。
Objective To investigate the changes of oxidative stress levels of atrial muscle cell and effect on atrial structure remodeling after programmed cell death 1(PD-1) gene knock-out in C57BL/6 mice.Methods The two kinds of mice were enrolled in the study:C57BL/6 control group (15 mice,including 6 males and 9 females ) and C57BL/6-PD-1 group (15 mice,including 6males and 9 females).The oxidative stress levels of atrial muscle cell and atrial myocardial fibrosis were observed for both groups .Results As compared with those in C57BL/6 control group,the oxidative stress levels of atrial muscle cell in PD-1 group were significantly increased ( P <0.05),meanwhile,Masson staining showed that atrial myocardial fibrosis was found in PD-1 group,however,which was not observed in C57BL/6 control group.Conclusion The increase of oxidative stress levels of atrial muscle cell presents after PD-1 gene knock-out in C57BL/6 mice,furthermore,which results in atrial structure remodeling of C57BL/6 mice.