CAJ | 학술논문

目的：观察通心络胶囊抑制NF-κB通路抑制糖尿病视网膜病变小鼠黏附分子的作用。方法 KK/Upj-Ay小鼠随机分为模型组、通心络高、中、低（生药4、2、1 g/kg）剂量组，另设C57BL /6小鼠对照组。灌胃给药3个月，测定血-视网膜屏障功能；流式测定血液中细胞间黏附分子-1（ ICAM-1）、血管细胞黏附分子-1（ VCAM-1）含量；Real Time PCR（ qPCR）和 Western blot法测定视网膜ICAM-1、VCAM-1和核因子-κB（ NF-κB）表达。结果通心络中、高剂组EB含量比模型组明显下降（ P ＜0．01）；通心络中、高剂组血清ICAM-1、VCAM-1含量显著下降（ P＜0．05）；通心络组ICAM-1、VCAM-1 mRNA及蛋白表达均显著下降（ P ＜0．01）；通心络中、高组核NF-κB表达显著下降（ P ＜0．05）；总NF-κB mRNA及蛋白表达在各组差异无统计学意义（ P ＞0．05）。结论通心络胶囊可抑制NF-κB通路抑制糖尿病视网膜病变小鼠黏附分子，改善糖尿病视网膜病变。
목적：관찰통심락효낭억제NF-κB통로억제당뇨병시망막병변소서점부분자적작용。방법 KK/Upj-Ay소서수궤분위모형조、통심락고、중、저（생약4、2、1 g/kg）제량조，령설C57BL /6소서대조조。관위급약3개월，측정혈-시망막병장공능；류식측정혈액중세포간점부분자-1（ ICAM-1）、혈관세포점부분자-1（ VCAM-1）함량；Real Time PCR（ qPCR）화 Western blot법측정시망막ICAM-1、VCAM-1화핵인자-κB（ NF-κB）표체。결과통심락중、고제조EB함량비모형조명현하강（ P ＜0．01）；통심락중、고제조혈청ICAM-1、VCAM-1함량현저하강（ P＜0．05）；통심락조ICAM-1、VCAM-1 mRNA급단백표체균현저하강（ P ＜0．01）；통심락중、고조핵NF-κB표체현저하강（ P ＜0．05）；총NF-κB mRNA급단백표체재각조차이무통계학의의（ P ＞0．05）。결론통심락효낭가억제NF-κB통로억제당뇨병시망막병변소서점부분자，개선당뇨병시망막병변。
Objective To investigate the effect of Tongxinluo capsule on adhesion molecules in mice with diabetic retinopathy through inhibiting NF-κB pathway.Methods KK/Upj-Ay mice were randomly divided into model group , Tongxinluo (low-dose,median-dose,high-dose) groups.C57BL/6 mice were selected as control group .The mice were given Tongxinluo intragastrically for 3 months.Then the retina barrier function was detected ,and contents of ICAM-1,VCAM-1 were determined by flow cytometry.The expression levels of ICAM-1,VCAM-1 and NF-κB in retina were detected by Real Time PCR ( qPCR) and Western Blot .Results The contents of EB in Tongxinluo median-dose and high-dose groups were obviously decreased ( P <0.01 );the serum levels of ICAM-1 and VCAM-1in Tongxinluo median-dose and high-dose groups were significantly decreased ( P <0.05);the expression levels of ICAM-1,VCAM-1 mRNA and protein in Tongxinluo groups were obviously decreased ( P <0.01);expression levels of NF-κB in Tongxinluo median-dose and high-dose groups were obviously decreased ( P <0.05),however,there were no significant differences in the expression levels of total NF-κB mRNA and protein among groups ( P >0.05).Conclusion Tongxinluo capsule can inhibit adhesion molecules in mice with diabetic retinopathy mice through inhibiting NF-κB pathway, as a result ,which can improve diabetic retinopathy .