CAJ | 학술논문

目的：检测膀胱尿路上皮癌组织中Pokemon蛋白的表达，分析其与临床病理特征间的关系。方法收集2010年3月~2013年8月宁波大学附属医院泌尿外科27例膀胱尿路上皮癌术中切除的癌、癌旁及临近正常组织，应用免疫印迹法(Western blot)检测标本中Pokemon蛋白的表达，并对其与膀胱癌的临床病理特征进行分析。结果 Western blot检测到Pokemon蛋白在膀胱尿路上皮癌组织中表达较高、在癌旁及正常组织中表达较低，差异有统计学意义(P<0.05)。膀胱癌组织中Pokemon蛋白表达与肿瘤病理分级有关，而与患者的性别、年龄、吸烟史及TNM临床分期无明显相关。结论 Pokemon蛋白表达和膀胱尿路上皮癌的发病及恶性程度有关，可能成为膀胱尿路上皮癌治疗的有效靶点。
목적：검측방광뇨로상피암조직중Pokemon단백적표체，분석기여림상병리특정간적관계。방법수집2010년3월~2013년8월저파대학부속의원비뇨외과27례방광뇨로상피암술중절제적암、암방급림근정상조직，응용면역인적법(Western blot)검측표본중Pokemon단백적표체，병대기여방광암적림상병리특정진행분석。결과 Western blot검측도Pokemon단백재방광뇨로상피암조직중표체교고、재암방급정상조직중표체교저，차이유통계학의의(P<0.05)。방광암조직중Pokemon단백표체여종류병리분급유관，이여환자적성별、년령、흡연사급TNM림상분기무명현상관。결론 Pokemon단백표체화방광뇨로상피암적발병급악성정도유관，가능성위방광뇨로상피암치료적유효파점。
Objective To explore the expression of Pokemon protein in bladder transitional cell carcinoma and its cor-relation with clinical features. Methods From March 2010 to August 2013, at Department of Urinary Surgery in Affili-ated Hospital of Ningbo University, 27 resection samples of patiens with bladder transitional cell carcinoma, including carcinoma tissue, paracancerous tissue and normal tissue were selected. Western blot was applied to detect the expres-sion of Pokemon protein in tissue and clinical-pathological features was analyzed. Results The result of Westen blot showed that the expression of Pokemon protein in bladder transitional cell carcinoma tissue was significantly higher than those of paracancerous tissue and normal tissue, the differences were statistically significant (P< 0.05). The ex-pression of Pokemon was related to different tumor degree, and no related to patient's gender, age, smoking history and TNM stages. Conclusion Pokemon protein is expressed in bladder transitional cell carcinoma and it may play a key role in the genesis of transitional cell carcinoma of bladder. It may be the therapeutic target of transitional cell carcino-ma of bladder in the future.