中国医药导报
中國醫藥導報
중국의약도보
CHINA MEDICAL HERALD
2014年
19期
4-6
,共3页
赵洁%张金山%李臻%冯潇%马斌芳
趙潔%張金山%李臻%馮瀟%馬斌芳
조길%장금산%리진%풍소%마빈방
胃饥饿素%胸腺%地塞米松%凋亡%小鼠
胃饑餓素%胸腺%地塞米鬆%凋亡%小鼠
위기아소%흉선%지새미송%조망%소서
Ghrelin%Thymus%Dexamethasone%Apoptosis%Mouse
目的：观察应激状态下胸腺组织内胃饥饿素(Ghrelin)的变化,为进一步探讨Ghrelin对胸腺细胞凋亡的影响提供±据。方法 BALB/c小鼠成年雄性32只,随机均分为4组院正常对照组、地塞米松(Dex)-6 h组、Dex-12 h组和Dex-24 h组,每组各8只。用Dex腹腔注射(5 mg/kg)诱发小鼠胸腺应激性萎缩模型,正常对照组给予等量生理盐水腹腔注射。用酶免疫分析法(EIA)检测各组小鼠胸腺组织匀浆中Ghrelin含量。结果与正常对照组相比,Dex-6 h组、Dex-12 h组和Dex-24 h组小鼠胸腺重量均有不同程度下降,其中Dex-24 h组最为明显；胸腺组织匀浆Ghrelin的EIA检测结果表明,与正常对照组比较,Dex-6 h组、Dex-12 h组和Dex-24 h组小鼠胸腺组织匀浆中Ghrelin含量均增加[(5.67±1.41)ng/g比(6.94±1.80)、(8.43±2.06)、(13.59±2.39)ng/g],其中Dex-24 h组与其他三组间比较,差异均有高度统计学意义(均P<0.01)。结论 Dex诱发小鼠胸腺应激性萎缩过程中Ghrelin表达上调,为进一步探讨Ghrelin对胸腺生物学功能的调节作用提供了实验±据。
目的：觀察應激狀態下胸腺組織內胃饑餓素(Ghrelin)的變化,為進一步探討Ghrelin對胸腺細胞凋亡的影響提供±據。方法 BALB/c小鼠成年雄性32隻,隨機均分為4組院正常對照組、地塞米鬆(Dex)-6 h組、Dex-12 h組和Dex-24 h組,每組各8隻。用Dex腹腔註射(5 mg/kg)誘髮小鼠胸腺應激性萎縮模型,正常對照組給予等量生理鹽水腹腔註射。用酶免疫分析法(EIA)檢測各組小鼠胸腺組織勻漿中Ghrelin含量。結果與正常對照組相比,Dex-6 h組、Dex-12 h組和Dex-24 h組小鼠胸腺重量均有不同程度下降,其中Dex-24 h組最為明顯；胸腺組織勻漿Ghrelin的EIA檢測結果錶明,與正常對照組比較,Dex-6 h組、Dex-12 h組和Dex-24 h組小鼠胸腺組織勻漿中Ghrelin含量均增加[(5.67±1.41)ng/g比(6.94±1.80)、(8.43±2.06)、(13.59±2.39)ng/g],其中Dex-24 h組與其他三組間比較,差異均有高度統計學意義(均P<0.01)。結論 Dex誘髮小鼠胸腺應激性萎縮過程中Ghrelin錶達上調,為進一步探討Ghrelin對胸腺生物學功能的調節作用提供瞭實驗±據。
목적：관찰응격상태하흉선조직내위기아소(Ghrelin)적변화,위진일보탐토Ghrelin대흉선세포조망적영향제공±거。방법 BALB/c소서성년웅성32지,수궤균분위4조원정상대조조、지새미송(Dex)-6 h조、Dex-12 h조화Dex-24 h조,매조각8지。용Dex복강주사(5 mg/kg)유발소서흉선응격성위축모형,정상대조조급여등량생리염수복강주사。용매면역분석법(EIA)검측각조소서흉선조직균장중Ghrelin함량。결과여정상대조조상비,Dex-6 h조、Dex-12 h조화Dex-24 h조소서흉선중량균유불동정도하강,기중Dex-24 h조최위명현；흉선조직균장Ghrelin적EIA검측결과표명,여정상대조조비교,Dex-6 h조、Dex-12 h조화Dex-24 h조소서흉선조직균장중Ghrelin함량균증가[(5.67±1.41)ng/g비(6.94±1.80)、(8.43±2.06)、(13.59±2.39)ng/g],기중Dex-24 h조여기타삼조간비교,차이균유고도통계학의의(균P<0.01)。결론 Dex유발소서흉선응격성위축과정중Ghrelin표체상조,위진일보탐토Ghrelin대흉선생물학공능적조절작용제공료실험±거。
Objective To observe the alteration of Ghrelin levels in stress-related thymic atrophy in the mice, and to provide the evidence for further exploring the effects of Ghrelin on thymic cell apoptosis. Methods 32 adult male BALB/c mice were randomly divided into four groups:normal control group, Dexamethasone (DEX)-6h treated group, DEX-12h treated group and DEX-24h treated group, 8 mice in each group. The intraperitoneal injection of Dexam-ethasone(5mg/kg) was used to induce the thymic atrophy in mice, and the mice in normal control group received nor-mal saline intraperitoneal injection. Enzyme-immunoassay (EIA) was used to evaluate the Ghrelin concentration in thy-mus homogenates from different experimental animals. Results Compared with normal control group, there were differ-ent degree of decline in DEX-6h treated group, DEX-12h treated group and DEX-24h treated group, with the most deleterious effect observed in the DEX-24h treated group. EIA test of Ghrelin in thymus tissue homogenate show that, compared with normal control group, the Ghrelin concentration in DEX-6h treated group, DEX-12h treated group and DEX-24h treated group thymus homogenates was gradually increased during the recovery after Dexamethasone injec-tion [(5.67±1.41) ng/g vs (6.94±1.80), (8.43±2.06), (13.59±2.39)ng/g], with the maximum level detected at the 24h af-ter Dexamethasone treatment, the differences were statistically significant (all P<0.01). Conclusion The expression level of Ghrelin was up-regulated in Dexamethasone-induced thymic atrophy model. This study lays the foundation for the further exploration of the possible role of Ghrelin in the regulation of the thymic biological function.
