贵州农业科学
貴州農業科學
귀주농업과학
GUIZHOU AGRICULTURAL SCIENCES
2014年
6期
109-112
,共4页
贺胜英%叶华%游玲
賀勝英%葉華%遊玲
하성영%협화%유령
大鲵%烂鳃病%病原菌%分离鉴定%药敏性
大鯢%爛鰓病%病原菌%分離鑒定%藥敏性
대예%란새병%병원균%분리감정%약민성
giant salamander(Andrias davidianus )%gill rot disease%pathogenic bacteria%isolation and identification%drug sensitivity
为了确定导致大鲵（Andrias davidianus ）烂鳃病的主要病原菌类型，从而为大鲵烂鳃病的确诊和科学防治提供参考，从细菌性感染病鲵的鳃、肝、肾及血液中分离到两株细菌，经形态结构、生理生化特征鉴定、16S rDNA 基因序列分析鉴定为蜡样芽孢杆菌（Bacillus cereus ）和栗褐芽孢杆菌（Bacillus badius ）。人工感染试验证实栗褐芽孢杆菌（Bacillus badius ）为主要致病菌。两种病原菌对恩诺沙星和环丙沙星比较敏感，可作为首选药物治疗该病。
為瞭確定導緻大鯢（Andrias davidianus ）爛鰓病的主要病原菌類型，從而為大鯢爛鰓病的確診和科學防治提供參攷，從細菌性感染病鯢的鰓、肝、腎及血液中分離到兩株細菌，經形態結構、生理生化特徵鑒定、16S rDNA 基因序列分析鑒定為蠟樣芽孢桿菌（Bacillus cereus ）和慄褐芽孢桿菌（Bacillus badius ）。人工感染試驗證實慄褐芽孢桿菌（Bacillus badius ）為主要緻病菌。兩種病原菌對恩諾沙星和環丙沙星比較敏感，可作為首選藥物治療該病。
위료학정도치대예（Andrias davidianus ）란새병적주요병원균류형，종이위대예란새병적학진화과학방치제공삼고，종세균성감염병예적새、간、신급혈액중분리도량주세균，경형태결구、생리생화특정감정、16S rDNA 기인서렬분석감정위사양아포간균（Bacillus cereus ）화률갈아포간균（Bacillus badius ）。인공감염시험증실률갈아포간균（Bacillus badius ）위주요치병균。량충병원균대은낙사성화배병사성비교민감，가작위수선약물치료해병。
The morphological structure and physiological and biochemical property of two trains isolated from gill,liver,kidney and blood of A.davidianus infected with gill rot disease were identified to determine main pathogenic bacteria types of A.davidianus gill rot disease and to provide a reference for diagnosis and scientific control of A.davidianus gill rot disease.Two stains were identified as B.cereus and Bacillus badius based on 16S rDNA gene sequencing analysis.The artificial infection test confirms that Bacillus cereus is the dominant pathogenic bacteria.Enrofloxacin and CPFX can be used as first choice drugs to control gill rot disease in artificial breeding of A.davidianus.
