CAJ | 학술논문

目的：研究人工牛黄抗槟榔诱导的口腔纤维化的效应及药理机制。方法培养小鼠口腔黏膜成纤维细胞、氯胺T法、明胶酶谱分析、qRT-PCR、ELISA和Western blotting等。结果人工牛黄可抑制槟榔提取物（Areca nut extract，ANE）诱导的口腔黏膜成纤维细胞的异常增殖及胶原累积。药理研究显示，人工牛黄可显著拮抗ANE诱导的MMP2活性下降和胶原基因的表达上调，抑制ANE诱导的TIMP-1和TIMP-2基因表达，及CTGF、TGFβ1、IL-6和TNFα的转录及释放，抑制ANE诱导的AKT、ERK/JNK MAPK和NF-κB信号通路的活化及氧化应激。结论人工牛黄具有良好的抗纤维化作用，是一种很有应用前景的抗口腔黏膜纤维化药物。
목적：연구인공우황항빈랑유도적구강섬유화적효응급약리궤제。방법배양소서구강점막성섬유세포、록알T법、명효매보분석、qRT-PCR、ELISA화Western blotting등。결과인공우황가억제빈랑제취물（Areca nut extract，ANE）유도적구강점막성섬유세포적이상증식급효원루적。약리연구현시，인공우황가현저길항ANE유도적MMP2활성하강화효원기인적표체상조，억제ANE유도적TIMP-1화TIMP-2기인표체，급CTGF、TGFβ1、IL-6화TNFα적전록급석방，억제ANE유도적AKT、ERK/JNK MAPK화NF-κB신호통로적활화급양화응격。결론인공우황구유량호적항섬유화작용，시일충흔유응용전경적항구강점막섬유화약물。
Objective In this study, we have investigated the anti-fibrotic activity of calculus bovis on areca nut-induced oral submucous fibrosis (OSF) in vitro. Methods Hydroxyproline assay, gelatin zymography, antioxidant assay, qRT-PCR, ELISA and western blotting were used. Results Calculus bovis could inhibit the ANE-induced abnormal growth and collagen accumulation of oral mucosal fibroblasts. Then we studied the underlying mechanism and found that calculus bovis (6.25 and 12.5μg/mL) could significantly augment the ANE-induced decrease of MMP2 activity, inhibit the ANE-induced expression of Col1A1, Col3A1, TIMP-1 and TIMP-2, the transcription and release of CTGF, TGFβ1, IL-6 and TNFα, the ANE-induced oxidant stress, and the ANE-induced activation of PI3K/AKT, ERK/JNK MAPK and NF-κB signal pathways. Conclusion Calculus bovis possesses excellent anti-fibrotic activity and is a promising drug for OSF.