中华行为医学与脑科学杂志
中華行為醫學與腦科學雜誌
중화행위의학여뇌과학잡지
CHINESE JOURNAL OF BEHAVIORAL MEDICINE AND BRAIN SCIENCE
2009年
9期
769-771
,共3页
叶翠飞%宋秋洁%艾厚喜%李林
葉翠飛%宋鞦潔%艾厚喜%李林
협취비%송추길%애후희%리림
淫羊藿黄酮%β-淀粉样肽%阿尔茨海默病%学习记忆
淫羊藿黃酮%β-澱粉樣肽%阿爾茨海默病%學習記憶
음양곽황동%β-정분양태%아이자해묵병%학습기억
Learning and memory%β-amyloid%Alzheimer's disease%Epimedium flavanoids
目的 观察淫羊藿黄酮对β-淀粉样肽(Aβ)拟阿尔茨海默病(AD)模型小鼠学习记忆能力的影响.方法 将ICR小鼠随机分为正常组、假手术组、模型组,淫羊藿黄酮小剂量(0.03 g/kg)、中剂量(0.1 g/kg)、大剂量(0.3 g/kg)组.灌胃给药14d后,行右侧脑室注射Aβ1-40造模,继续给药7 d,进行Morris水迷宫、避暗实验和自主活动实验,观察小鼠学习记忆能力.结果 Aβ1-40侧脑室注射模型小鼠出现学习记忆障碍.淫羊藿黄酮中、大剂量组在Moms水迷宫实验中逃避潜伏期[(40.12±4.15)s.(34.99±5.49)s]和游泳距离[(648.36±88.42)cm,(781.57±104.41)cm]明显缩短.与模型组逃避潜伏期(65.45±5.15)s,游泳距离(1142.66±96.80)em比较,差异具有显著性(P<0.01),在避暗实验中可延长模型小鼠的潜伏期[(255.40±11.00)s,(257.46±19.50)s]并降低其错误次数[(0.80±0.14)次,(0.77±0.17)次],与模型组潜伏期[(196.27±25.47)s],错误次数[(1.47±0.31)次]比较,差异有显著性(P<0.05).在自主活动实验中,各组之间差异无显著性(P>0.05).结论 淫羊藿黄酮能明显提高Aβ脑室注射致AD小鼠模型的学习记忆能力.
目的 觀察淫羊藿黃酮對β-澱粉樣肽(Aβ)擬阿爾茨海默病(AD)模型小鼠學習記憶能力的影響.方法 將ICR小鼠隨機分為正常組、假手術組、模型組,淫羊藿黃酮小劑量(0.03 g/kg)、中劑量(0.1 g/kg)、大劑量(0.3 g/kg)組.灌胃給藥14d後,行右側腦室註射Aβ1-40造模,繼續給藥7 d,進行Morris水迷宮、避暗實驗和自主活動實驗,觀察小鼠學習記憶能力.結果 Aβ1-40側腦室註射模型小鼠齣現學習記憶障礙.淫羊藿黃酮中、大劑量組在Moms水迷宮實驗中逃避潛伏期[(40.12±4.15)s.(34.99±5.49)s]和遊泳距離[(648.36±88.42)cm,(781.57±104.41)cm]明顯縮短.與模型組逃避潛伏期(65.45±5.15)s,遊泳距離(1142.66±96.80)em比較,差異具有顯著性(P<0.01),在避暗實驗中可延長模型小鼠的潛伏期[(255.40±11.00)s,(257.46±19.50)s]併降低其錯誤次數[(0.80±0.14)次,(0.77±0.17)次],與模型組潛伏期[(196.27±25.47)s],錯誤次數[(1.47±0.31)次]比較,差異有顯著性(P<0.05).在自主活動實驗中,各組之間差異無顯著性(P>0.05).結論 淫羊藿黃酮能明顯提高Aβ腦室註射緻AD小鼠模型的學習記憶能力.
목적 관찰음양곽황동대β-정분양태(Aβ)의아이자해묵병(AD)모형소서학습기억능력적영향.방법 장ICR소서수궤분위정상조、가수술조、모형조,음양곽황동소제량(0.03 g/kg)、중제량(0.1 g/kg)、대제량(0.3 g/kg)조.관위급약14d후,행우측뇌실주사Aβ1-40조모,계속급약7 d,진행Morris수미궁、피암실험화자주활동실험,관찰소서학습기억능력.결과 Aβ1-40측뇌실주사모형소서출현학습기억장애.음양곽황동중、대제량조재Moms수미궁실험중도피잠복기[(40.12±4.15)s.(34.99±5.49)s]화유영거리[(648.36±88.42)cm,(781.57±104.41)cm]명현축단.여모형조도피잠복기(65.45±5.15)s,유영거리(1142.66±96.80)em비교,차이구유현저성(P<0.01),재피암실험중가연장모형소서적잠복기[(255.40±11.00)s,(257.46±19.50)s]병강저기착오차수[(0.80±0.14)차,(0.77±0.17)차],여모형조잠복기[(196.27±25.47)s],착오차수[(1.47±0.31)차]비교,차이유현저성(P<0.05).재자주활동실험중,각조지간차이무현저성(P>0.05).결론 음양곽황동능명현제고Aβ뇌실주사치AD소서모형적학습기억능력.
Objective To investigate the effects of Epimedium flavanoids(EF)on learning and memory ability in Alzheimer's disease(AD)mouse model induced by β-amyloid(Aβ)lateral ventricle injection. Methods ICR mice were divided into normal group, sham operation group, model group, positive control drug(Ibuprofen)group, EF low dose(0.03 g/kg),middle dose(0.1 g/kg)and high dose(0.3 g/kg)groups. The model was induced by injecting Aβ1-40 into right lateral ventricle of mice. In the treatment groups, EF was introgastrically administrated 14 d before injection and 7 d after injection. The learning and memory ability was determined by Morris water maze, step through and spontaneous locomotor activity tests. Results The function of learning-memory was signifcantly decreased in mouse model induced by Aβ lateral ventricle injection. Administration of middle and high dose EF significantly decreased the escape latency[(40.12±4.15)s,(34.99±5.49)s]and swimming distanee[(648.36±88.42)cm,(781.57±104.41)cm]than that of the model group(65.45±5.15)s,(1142.66±96.80)cm.P＜0.01)in Moms water maze test,and prolonged the latent period[(255.40±11.00)s,(257.46±19.50)s]and decreased the error times(0.80±0.14,0.77±0.17)in step-through test significantly, compared with the model mice[(196.27±25.47)s,(1.47±0.31)](P＜0.05).In the spontaneous locomotor activity experiment, no obvious difference was found among all groups(P＞0.05).Conclusion EF significantly improved the learning and memory ability in AD mouse model induced by A∞1-40 lateral ventricle injection.
