CAJ | 학술논문

目的观察碱性成纤维细胞生长因子(basic fibroblast growth factor,bFGF)转基因治疗对放射性脑损伤(RIB)模型鼠学习记忆能力的影响,为探索治疗RIB的新途径提供实验基础.方法 PCR法克隆出bFGF基因片段,酶切后连接法构建bFGF-pcDNA3.1(+)质粒.分组采用直线加速器对Sprague-Dawley大鼠进行全脑单次25Gy照射,bFGF转基因治疗组采用侧脑室注射bFGF-pcDNA3.1(+)质粒,并设未照射组为对照.在照射前及照射后7d,20d,60d分别进行Moms水迷宫试验,分析各组大鼠行为学检测结果.采用平均逃避潜伏期和搜索策略2个指标评价大鼠学习记忆能力,同时观察各组脑组织病理变化.结果各组大鼠放射前平均逃避潜伏期和搜索策略得分无统计学差异,bFGF治疗组、照射组和对照组在照射后不同时间点平均潜伏期和搜索策略差异有显著性(P<0.05)).bFGF治疗组在放射后7 d、20d平均逃避潜伏期[(68.42±11.35)s、(50.87±12.06)s]和搜索策略[(16.89±2.08)%、(19.98±2.14)%]明显下降,60d时潜伏期[(40.28±10.87)s]和搜索策略[(26.42±2.59)%]恢复至照射前水平,放射组60d后仍没有恢复.照射组病理检查显示海马和皮层神经元轻度变性.胞体萎缩,白质区域较对照组呈现组织结构梳松、血管周隙扩大等表现;bFGF治疗组病变程度明显较照射组轻.结论 25Gy射线照射可降低大鼠学习记忆能力,bFGF转基因治疗可逆转放射造成的大鼠学习记忆能力下降.
목적 관찰감성성섬유세포생장인자(basic fibroblast growth factor,bFGF)전기인치료대방사성뇌손상(RIB)모형서학습기억능력적영향,위탐색치료RIB적신도경제공실험기출.방법 PCR법극륭출bFGF기인편단,매절후련접법구건bFGF-pcDNA3.1(+)질립.분조채용직선가속기대Sprague-Dawley대서진행전뇌단차25Gy조사,bFGF전기인치료조채용측뇌실주사bFGF-pcDNA3.1(+)질립,병설미조사조위대조.재조사전급조사후7d,20d,60d분별진행Moms수미궁시험,분석각조대서행위학검측결과.채용평균도피잠복기화수색책략2개지표평개대서학습기억능력,동시관찰각조뇌조직병리변화.결과 각조대서방사전평균도피잠복기화수색책략득분무통계학차이,bFGF치료조、조사조화대조조재조사후불동시간점평균잠복기화수색책략차이유현저성(P<0.05)).bFGF치료조재방사후7 d、20d평균도피잠복기[(68.42±11.35)s、(50.87±12.06)s]화수색책략[(16.89±2.08)%、(19.98±2.14)%]명현하강,60d시잠복기[(40.28±10.87)s]화수색책략[(26.42±2.59)%]회복지조사전수평,방사조60d후잉몰유회복.조사조병리검사현시해마화피층신경원경도변성.포체위축,백질구역교대조조정현조직결구소송、혈관주극확대등표현;bFGF치료조병변정도명현교조사조경.결론 25Gy사선조사가강저대서학습기억능력,bFGF전기인치료가역전방사조성적대서학습기억능력하강.
Objective To study the effects of basic fibroblast growth factor(bFGF)transfection therapy on learning and memory ability of radiation brain injuries rat model.Methods The bFGF gene was cloned by PCR method and linked to pc-DNA3.1(+)plasmid.Sprague-Dawley rats were grouped to 3 groups and received different treatment.The rats of bFGF group were injected bFGF-pcDNA3.1(+)plasmid into lateral ventricle after radiation and the rats of radiation group were only received single 25 Gy radiation by linear accelerator.Control group was set for contrast.Before radiation and 7,20,60 days after radiation,Moms maze test were taken to study the learning and memory ability of rats in each group.Average escape latency(AEL)and search strategy(SS)were scaled and analyzed.Pathology examinations were taken in every group.Results No statistic differences of AEL and SS were found in each group before radiation.After radiation,learning and memory ability of rats in each group were dramatically changed(P＜0.05).The scales of ALE in bFGF group were decreased after 7 and 20 days[(68.42±11.35)s,(50.87±12.06)s]and so were the scales of SS[(16.89±2.08)%,(19.98±2.14)%]and recovered after 60 days[AEL:(40.28±10.87)s;SS:(26.42±2.59)%]but no recoveries were found in radiation and control group after 60 days of radiation.Neurons were atrophic degenerated and whiter matter were loosen in radiation group but the pathologic changes were lessen in bFGF groups.Conclusion The impairment of learning and memory ability caused by 25 Gy radiation can be reversed by bFGF transgenic therapy.