CAJ | 학술논문

目的探讨加味温胆汤对抑郁模型大鼠海马钙调蛋白激酶(CaMK)通路、丝裂原活化蛋白激酶(MAPK)通路的整体调节作用.方法以孤养结合慢性不可预见性应激抑郁模型大鼠为研究对象,在CaMK、MAPK信号转导通路上关键因子mRNA/蛋白表达及信号通路交汇点CREB1 mRNA表达检测的基础上,采用相关分析方法,探讨CaMK、MAPK级联信号转导通路上关键因子CaMKⅡ、RSK蛋白与信号通路交汇点CREB1mRNA的相关性.结果 ①双向相关分析:模型组、中药组、西药组大鼠海马CaMKⅡ蛋白与CREB1mRNA表达的相关系数分别为r=-0.502(P＜0.01),r=-0.643,(P＜0.01),r=-0.408 (P＜0.05)；模型组、中药组、西药组大鼠海马RSK蛋白与CREB1 mRNA表达的相关系数分别为r=0.550(P＜0.01),r=0.687(P＜0.01),r=0.407(P＜0.01).②回归分析(强行投入法):模型组、中药组、西药组大鼠海马CaMKⅡ蛋白(负向)和RSK(正向)与CREB1mRNA表达的回归系数分别为:R2 =0.472,F=12.983(P＜0.01),R2=0.666,F=28.961(P＜0.01),R2 =0.356,F=8.004(P＜0.01).结论加味温胆汤可通过整体调节CaMK、MAPK信号转导通路,进而上调CREB1mRNA表达发挥抗抑郁效应.
목적 탐토가미온담탕대억욱모형대서해마개조단백격매(CaMK)통로、사렬원활화단백격매(MAPK)통로적정체조절작용.방법 이고양결합만성불가예견성응격억욱모형대서위연구대상,재CaMK、MAPK신호전도통로상관건인자mRNA/단백표체급신호통로교회점CREB1 mRNA표체검측적기출상,채용상관분석방법,탐토CaMK、MAPK급련신호전도통로상관건인자CaMKⅡ、RSK단백여신호통로교회점CREB1mRNA적상관성.결과 ①쌍향상관분석:모형조、중약조、서약조대서해마CaMKⅡ단백여CREB1mRNA표체적상관계수분별위r=-0.502(P＜0.01),r=-0.643,(P＜0.01),r=-0.408 (P＜0.05)；모형조、중약조、서약조대서해마RSK단백여CREB1 mRNA표체적상관계수분별위r=0.550(P＜0.01),r=0.687(P＜0.01),r=0.407(P＜0.01).②회귀분석(강행투입법):모형조、중약조、서약조대서해마CaMKⅡ단백(부향)화RSK(정향)여CREB1mRNA표체적회귀계수분별위:R2 =0.472,F=12.983(P＜0.01),R2=0.666,F=28.961(P＜0.01),R2 =0.356,F=8.004(P＜0.01).결론 가미온담탕가통과정체조절CaMK、MAPK신호전도통로,진이상조CREB1mRNA표체발휘항억욱효응.
Objective To explore the effect of Jiawei Wendan decoction on whole regulating function of CaMK/MAPK signal pathway in hippocampus of depression model rats.Methods Isolated depression model rats as research object were living unpredictable chronic stress.Based on the examination of mRNA/protein expression which was the key factor of the signal pathway of CaMK/MAPK and the intersection CREB1 mRNA expression,the relationship between CaMK Ⅱ/RSK protein expression and the CREB1 mRNA expression were analyzed with relation analysis method.Results ① Bidirectional correlation analysis:the respective coefficient (r value) of CaMK Ⅱ protein expression and the CREB1mRNA expression in hippocampus of the model group,Chinese medicine treatment group and western medicine treatnent group were-0.502 (P ＜ 0.01),-0.643 (P ＜ 0.01),-0.408 (P＜ 0.05) ;the respective coefficient(r value) of RSK protein expression and the CREB1 mRNA expression in hippocampus of the model group,Chinese medicine treatment group and western medicine treatment group were 0.550 (P ＜ 0.01),0.687 (P ＜ 0.0 l),0.407 (P ＜ 0.01).②Regression analysis:the respective regression coefficient of CaMK Ⅱ protein (positive direction),RSK protein (negative direction) and CREB1 mRNA expression in the model group,Chinese nedicine treatment group and western medicine treatment group were R 2 =0.472,F =12.983(P＜0.01),R2=0.666,F=28.961(P＜0.01),R2=0.356,F=8.004(P＜0.01).Conclusion The anti-depressant effect mechanism of Jiawei Wendan decoction is to regulate the whole function of the CaMK and MAPK signal pathway and then further up-regulate CREB1 mRNA expression.