CAJ | 학술논문

目的：研究高效液相色谱法测定氯霉素醇溶液中氯霉素的含量。方法采用高效液相色谱法，以Eclipse XDB-C18(4.6 mm×250 mm，5μm)为色谱柱；流动相为0.01 mol/L庚烷磺酸钠缓冲液(取磷酸二氢钾6.8 g，用0.01 mol/L庚烷磺酸钠溶液溶解并稀释至1000 mL，再加三乙胺5 mL，混匀，用0.01 mol/L磷酸调节pH值至2.5)-甲醇(68：32)；流速1.0 mL/min，检测波长277 nm，进样量10μL。结果氯霉素在60~160μg/mL范围内与峰面积呈良好的线性关系，回归方程为A=17.79C+17.13，r=0.9995；平均回收率为100.24%，RSD=1.26%。结论本方法简便快速，定量准确，结果稳定、重现性好，可用于氯霉素醇溶液中氯霉素的含量测定。
목적：연구고효액상색보법측정록매소순용액중록매소적함량。방법채용고효액상색보법，이Eclipse XDB-C18(4.6 mm×250 mm，5μm)위색보주；류동상위0.01 mol/L경완광산납완충액(취린산이경갑6.8 g，용0.01 mol/L경완광산납용액용해병희석지1000 mL，재가삼을알5 mL，혼균，용0.01 mol/L린산조절pH치지2.5)-갑순(68：32)；류속1.0 mL/min，검측파장277 nm，진양량10μL。결과록매소재60~160μg/mL범위내여봉면적정량호적선성관계，회귀방정위A=17.79C+17.13，r=0.9995；평균회수솔위100.24%，RSD=1.26%。결론본방법간편쾌속，정량준학，결과은정、중현성호，가용우록매소순용액중록매소적함량측정。
Objective To establish a method for the determination of Chloramphenicol in Chloramphenicol Alcoholic Solution by HPLC. Methods HPLC was used for quantitative analysis. Eclipse XDB-C18 (4.6 mmí250 mm, 5μm) was used, 0.01 mol/L heptane sodium sulfonate buffer solution (1000 mL 0.01 mol/L heptane sodium sulfonate buffer solu-tion consisted of 6.8 g dipotassium phosphate, 5 mL triethylamine and 0.01 mol/L heptanes sulfonic acid sodium solu-tion, adjusted pH to 2.5 by phosphoric acid solution) - methanol (68:32) was used as mobile phases, the flow rate was 1.0 mL/min, the detective wavelength was at 277 nm and the injection volume was 10 μL. Results The calibration curve was linear in the range of 60-160 μg/mL for Cholramphenicol, and the equation of linear regression was A=17.79C+17.13 (r = 0.9995); the average recovery rate was 100.24% and RSD was 1.26%. Conclusion The method is simple, accurate with good reproducibility and stability, and can be used for the quality control of the Chloramphenicol Alcoholic Solution.