CAJ | 학술논문

目的：制备生物色谱柱，用于分离或测定药物成分。方法采用3-氨基丙基三甲氧基硅烷(APTS)与硅胶反应生成氨基功能化的活化硅胶，并与牛血清蛋白(BSA)共价结合形成BSA键合固定相，匀浆装柱制备成BSA生物色谱柱，以阿司匹林(Aspirin，ASP)为分析对象考察BSA固定相的结合性能和分离性能。结果 BSA结合率为3.69μmol/g，ASP在BSA键合固定相色谱柱上的最大结合量为74 mg/g，经BSA色谱柱测得阿司匹林肠溶片的含量为14.65μg/片，标示量为107.8%。结论这表明BSA与硅胶载体成功结合形成BSA键合固定相，所制备的BSA生物色谱柱具有良好的结合性能与分离性能。
목적：제비생물색보주，용우분리혹측정약물성분。방법채용3-안기병기삼갑양기규완(APTS)여규효반응생성안기공능화적활화규효，병여우혈청단백(BSA)공개결합형성BSA건합고정상，균장장주제비성BSA생물색보주，이아사필림(Aspirin，ASP)위분석대상고찰BSA고정상적결합성능화분리성능。결과 BSA결합솔위3.69μmol/g，ASP재BSA건합고정상색보주상적최대결합량위74 mg/g，경BSA색보주측득아사필림장용편적함량위14.65μg/편，표시량위107.8%。결론저표명BSA여규효재체성공결합형성BSA건합고정상，소제비적BSA생물색보주구유량호적결합성능여분리성능。
Objective To prepare biological chromatographic column to separate and determine the ingredients of drugs. Methods Silica gel was reacted with 3-aminopropyl trimethoxylsilane to form amine functionalized activated sil-ica gel, which was covalent binding with BSA to synthesize BSA bonded stationary phase. Then BSA bonded stationary phase was packed column with homogenization, thus BSA biological chromatographic column was prepared. Aspirin was taken as a detective object to examine the combination performance and separation performance of BSA bonded sta-tionary phase. Results It came to that, the combined rate was 3.69 μmol/g, the biological chromatographic column was 74 mg/g, the amount of Aspirin in per tablet was 14.65 μg and the labeled amount was 107.8% detected by BSA bio-logical chromatographic column. Conclusion All of above shows that BSA bonded stationary phase is synthesized suc-cessfully, and the BSA biological chromatographic column has good combination property and separation property.