中国医药导报
中國醫藥導報
중국의약도보
CHINA MEDICAL HERALD
2014年
16期
9-11,19
,共4页
竹荪%水提物%LLC%增殖%抑制
竹蓀%水提物%LLC%增殖%抑製
죽손%수제물%LLC%증식%억제
Dictyophora indusiata%Aqueous extract%Lewis lung cancer%Proliferation%Inhibition
目的：探讨竹荪水提物对肺癌细胞的抑制效应。方法选取路易斯肺癌细胞(LLC)为靶细胞，接种于48孔或96孔板，接种密度分别为2×104/孔和1×104/孔，加入不同浓度的竹荪水提物院0、1、2 mg/mL，培养1~3 d，分别采用镜下观察、细胞计数试剂盒(CCK-8)、流式细胞学和细胞实时分析系统(RTCA)检测细胞生长状态。结果镜下直接观察，与对照组比较，竹荪水提物处理组LLC细胞数量明显较少，高浓度组细胞生长状态不佳；CCK-8检测结果显示，高浓度组OD450为0.24，仅为对照组的一半(0.59)，细胞增殖受到明显抑制，抑制率为59%(P<0.05)；流式细胞学检测显示，经竹荪水提物处理后，LLC早期凋亡比例由6%上升到48%，凋亡及死亡细胞比例由41%上升到68%；RTCA实时监测数据显示，竹荪水提物高浓度处理组LLC生长的电阻值为0，低浓度组为0.4，对照组为3.7，细胞贴壁生长状态呈剂量-效应关系。结论竹荪水提物可以抑制LLC生长。
目的：探討竹蓀水提物對肺癌細胞的抑製效應。方法選取路易斯肺癌細胞(LLC)為靶細胞，接種于48孔或96孔闆，接種密度分彆為2×104/孔和1×104/孔，加入不同濃度的竹蓀水提物院0、1、2 mg/mL，培養1~3 d，分彆採用鏡下觀察、細胞計數試劑盒(CCK-8)、流式細胞學和細胞實時分析繫統(RTCA)檢測細胞生長狀態。結果鏡下直接觀察，與對照組比較，竹蓀水提物處理組LLC細胞數量明顯較少，高濃度組細胞生長狀態不佳；CCK-8檢測結果顯示，高濃度組OD450為0.24，僅為對照組的一半(0.59)，細胞增殖受到明顯抑製，抑製率為59%(P<0.05)；流式細胞學檢測顯示，經竹蓀水提物處理後，LLC早期凋亡比例由6%上升到48%，凋亡及死亡細胞比例由41%上升到68%；RTCA實時鑑測數據顯示，竹蓀水提物高濃度處理組LLC生長的電阻值為0，低濃度組為0.4，對照組為3.7，細胞貼壁生長狀態呈劑量-效應關繫。結論竹蓀水提物可以抑製LLC生長。
목적：탐토죽손수제물대폐암세포적억제효응。방법선취로역사폐암세포(LLC)위파세포，접충우48공혹96공판，접충밀도분별위2×104/공화1×104/공，가입불동농도적죽손수제물원0、1、2 mg/mL，배양1~3 d，분별채용경하관찰、세포계수시제합(CCK-8)、류식세포학화세포실시분석계통(RTCA)검측세포생장상태。결과경하직접관찰，여대조조비교，죽손수제물처리조LLC세포수량명현교소，고농도조세포생장상태불가；CCK-8검측결과현시，고농도조OD450위0.24，부위대조조적일반(0.59)，세포증식수도명현억제，억제솔위59%(P<0.05)；류식세포학검측현시，경죽손수제물처리후，LLC조기조망비례유6%상승도48%，조망급사망세포비례유41%상승도68%；RTCA실시감측수거현시，죽손수제물고농도처리조LLC생장적전조치위0，저농도조위0.4，대조조위3.7，세포첩벽생장상태정제량-효응관계。결론죽손수제물가이억제LLC생장。
Objective To study the inhibition effect of aqueous extract from Dictyophora indusiata on Lewis lung cancer cell. Methods LLCs were seeded in 48-well or 96-well plate, with each well containing 2í104/well and 1í104/well, respectively. Different concentration aqueous extract from Dictyophora indusiata (DI) (0, 1, 2 mg/mL) were added into culture system, after 1-3 days, the cell status, proliferation and apoptosis were detected by microscopy, CCK-8 kit, flow cytometry and real time cell analysis system. Results Comparison to control group, the cell number of DI treated group obviously decreased. Furthermore, the cell status was weak in high dose group. The OD450 value of high dose group (0.24) was only half of the control group (0.59) in the CCK-8 experiment, and it indicated that the cell prolifera-tion was inhibited with the inhibited rate of 59% (P<0.05). In flow cytometry analysis, the apoptosis in early stage of control group was 6%, while it increased to 48% in DI treated group. The total death cell from 41% increased to 68%after DI treated. The results of RTCA showed that the proliferation of LLC obviously inhibited by DI, with the CI was 0 in high dose group and 0.4 in low dose group, and 3.7 in control group, and there was dose-effect relationship. Con-clusion DI aqueous extract has the inhibition effect on LLC.
