CAJ | 학술논문

目的：动态观察梗阻性黄疸小鼠血浆MFG-E8水平，以及肝脏、脾脏、淋巴结、胸腺的MFG-E8 mRNA和蛋白的表达情况，探讨其在梗阻性黄疸中的意义。方法建立梗阻性黄疸小鼠模型，分为假手术组、胆总管结扎组，采用蛋白免疫印迹法检测梗阻性黄疸小鼠第3、7、14天血浆中 MFG-E8蛋白水平；RT-PCR检测肝脏、脾脏、淋巴结、胸腺中 MFG-E8 mRNA的表达变化；采用蛋白免疫印迹法检测上述组织中 MFG-E8的蛋白表达水平。结果梗阻性黄疸小鼠血浆中 MFG-E8蛋白水平在每个时相点均较假手术组明显降低（P＜0．05）；肝脏、脾脏、淋巴结、胸腺组织中 MFG-E8 mRNA表达明显降低（P＜0．05），同时，肝脏、脾脏、淋巴结、胸腺组织中 MFG-E8蛋白表达水平亦明显下降（P＜0．05）。结论 MFG-E8在梗阻性黄疸免疫组织中的表达明显减少，可能对梗阻性黄疸后组织凋亡细胞的清除产生重要的影响。
목적：동태관찰경조성황달소서혈장MFG-E8수평，이급간장、비장、림파결、흉선적MFG-E8 mRNA화단백적표체정황，탐토기재경조성황달중적의의。방법건립경조성황달소서모형，분위가수술조、담총관결찰조，채용단백면역인적법검측경조성황달소서제3、7、14천혈장중 MFG-E8단백수평；RT-PCR검측간장、비장、림파결、흉선중 MFG-E8 mRNA적표체변화；채용단백면역인적법검측상술조직중 MFG-E8적단백표체수평。결과경조성황달소서혈장중 MFG-E8단백수평재매개시상점균교가수술조명현강저（P＜0．05）；간장、비장、림파결、흉선조직중 MFG-E8 mRNA표체명현강저（P＜0．05），동시，간장、비장、림파결、흉선조직중 MFG-E8단백표체수평역명현하강（P＜0．05）。결론 MFG-E8재경조성황달면역조직중적표체명현감소，가능대경조성황달후조직조망세포적청제산생중요적영향。
Objective To dynamically observe the plasma levels of MFG-E8 in mice with obstructive jaundice and the expression of MFG-E8 mRNA and protein in liver,spleen,lymph nodes and thymus,and to investigate their significance in obstructive jaun-dice.Methods The mice models of obstructive jaundice were established and divided into the sham operation group and the common bile duct ligation group.Western blot was adopted to detect plasma MFG-E8 protein expression in obstructive jaundice mice on 3,7, 14 d;the MFG-E8 mRNA expression changes in liver,spleen,lymph nodes and thymus tissue were detected by RT-PCR;the MFG-E8 protein expression in the above organizations was detected by Western blot.Results Plasma MFG-E8 protein levels at each time point in the obstructive jaundice mice were significantly lower than those in the control group (P<0.05);MFG-E8 mRNA expres-sion in liver,spleen,lymph nodes and thymus tissue was significantly decreased (P<0.05),meanwhile the MFG-E8 protein levels in liver,spleen,lymph nodes and thymus tissue were significantly decreased (P<0.05).Conclusion the expression of MFG-E8 in obstructive jaundice immune tissue is significantly decreased,which may have a significant impact on the clearance of apoptotic cells in obstructive jaundice.