CAJ | 학술논문

目的：探讨细胞角蛋白13（CK13）在鼻息肉上皮细胞增生分化过程中的作用。方法选取慢性鼻-鼻窦炎（伴鼻息肉）患者60例。其中 A组30例患者，术前未应用糖皮质激素直接进行手术治疗，术中取下鼻息肉组织；B组30例患者应用糖皮质激素类药物治疗3～5 d后手术治疗，术中取下鼻息肉组织。C组10例为正常中鼻甲组织对照组。采用免疫组化（二步法）检测标本中CK13的表达。结果 A组CK13的阳性表达率高于B组，差异有统计学意义（χ2＝11．482，P＝0．009），C组正常中鼻甲组织上皮细胞未见CK13的阳性表达。结论（1）CK13在鼻息肉组织中不同上皮表达程度的差异，反映了 CK13在鼻息肉在发生发展过程中的动态变化；使用糖皮质激素治疗后鼻息肉组织中 CK13蛋白表达有所下降，说明糖皮质激素对鼻息肉的增生、分化有抑制作用。
목적：탐토세포각단백13（CK13）재비식육상피세포증생분화과정중적작용。방법선취만성비-비두염（반비식육）환자60례。기중 A조30례환자，술전미응용당피질격소직접진행수술치료，술중취하비식육조직；B조30례환자응용당피질격소류약물치료3～5 d후수술치료，술중취하비식육조직。C조10례위정상중비갑조직대조조。채용면역조화（이보법）검측표본중CK13적표체。결과 A조CK13적양성표체솔고우B조，차이유통계학의의（χ2＝11．482，P＝0．009），C조정상중비갑조직상피세포미견CK13적양성표체。결론（1）CK13재비식육조직중불동상피표체정도적차이，반영료 CK13재비식육재발생발전과정중적동태변화；사용당피질격소치료후비식육조직중 CK13단백표체유소하강，설명당피질격소대비식육적증생、분화유억제작용。
Objective To research the role of cytokeratin13(CK13)during hyperplasia and differentiation process of epithelial cells in nasal polyps.Methods 60 cases of chronic rhinosinusitis (complicating nasal polyps)were selected.Among them,the group A(30 cases)was directly performed the operation without using preoperative glucocorticoid and the nasal polyp tissue was taken during operation;the C group(30 cases)was performed the operation after using glucocorticoid for 3-5 d and the nasal polyp tissue was intraoperatively taken.Other 10 cases of normal concha nasalis media tissue were taken as the control group.The immunohisto-chemical method(two step method)was adopted to detect the expression of CK13.The obtained data were statistically analyzed by SPSS 11.5.Results The positive expression of CK13 in the group A was higher than that in the group B with statistical difference between them(χ2=11.482,P=0.009).The epithelial cells of normal concha nasalis media tissue in the group C had no positive ex-pression of CK13.Conclusion (1)The difference of CK13 expression in different epithelias of nasal polyps reflects the dynamic change of nasal polyps during the development and progress process;(2)the expression of CK13 is decreased after using glucocorti-coid,which indicating that glucocorticoid has the inhibiting effect on the hyperplasia and differentiation of nasal polyps.