CAJ | 학술논문

目的：探讨新生儿窒息后缺氧缺血性脑病(HIE)患儿血清神经元特异性烯醇化酶(NSE)的动态变化及意义。方法收集2005年1月~2012年12月住院治疗的足月窒息新生儿75例(窒息组)和健康足月新生儿50例(对照组)，采用酶联免疫吸附试验法检测两组新生儿出生后1、3、7 d时血清NSE浓度变化，并分析其变化与窒息后HIE的关系。结果出生后1、3、7 d，窒息组新生儿血清NSE浓度均明显高于对照组[(44.50±12.08)μg/Lvs(15.59±6.25)μg/L，(42.65±12.43)μg/Lvs (15.38±5.84)μg/L，(37.90±9.50)μg/Lvs (13.92±5.37)μg/L]，差异有统计学意义(P<0.05)。重度窒息新生儿血清NSE浓度明显高于轻度窒息新生儿[(58.95±14.85)μg/Lvs (29.15±9.32)μg/L，(62.95±19.13)μg/Lvs (22.35±5.75)μg/L，(57.35±13.75)μg/Lvs (18.45±5.25)μg/L]，差异有统计学意义(P<0.05)。出生后1、3、7 d，HIE新生儿19例血清NSE浓度逐渐升高，而MRI正常新生儿56例血清NSE浓度逐渐下降，HIE新生儿血清NSE浓度均明显高于MRI正常新生儿[(59.84±15.09)μg/Lvs (32.95±12.86)μg/L，(63.95±19.21)μg/L vs (25.73±6.75)μg/L，(68.25±19.79)μg/L vs (19.62±5.94)μg/L]，差异有统计学意义(P<0.05)。结论监测血清NSE浓度动态变化有助于早期辅助判断窒息新生儿HIE。
목적：탐토신생인질식후결양결혈성뇌병(HIE)환인혈청신경원특이성희순화매(NSE)적동태변화급의의。방법수집2005년1월~2012년12월주원치료적족월질식신생인75례(질식조)화건강족월신생인50례(대조조)，채용매련면역흡부시험법검측량조신생인출생후1、3、7 d시혈청NSE농도변화，병분석기변화여질식후HIE적관계。결과출생후1、3、7 d，질식조신생인혈청NSE농도균명현고우대조조[(44.50±12.08)μg/Lvs(15.59±6.25)μg/L，(42.65±12.43)μg/Lvs (15.38±5.84)μg/L，(37.90±9.50)μg/Lvs (13.92±5.37)μg/L]，차이유통계학의의(P<0.05)。중도질식신생인혈청NSE농도명현고우경도질식신생인[(58.95±14.85)μg/Lvs (29.15±9.32)μg/L，(62.95±19.13)μg/Lvs (22.35±5.75)μg/L，(57.35±13.75)μg/Lvs (18.45±5.25)μg/L]，차이유통계학의의(P<0.05)。출생후1、3、7 d，HIE신생인19례혈청NSE농도축점승고，이MRI정상신생인56례혈청NSE농도축점하강，HIE신생인혈청NSE농도균명현고우MRI정상신생인[(59.84±15.09)μg/Lvs (32.95±12.86)μg/L，(63.95±19.21)μg/L vs (25.73±6.75)μg/L，(68.25±19.79)μg/L vs (19.62±5.94)μg/L]，차이유통계학의의(P<0.05)。결론감측혈청NSE농도동태변화유조우조기보조판단질식신생인HIE。
Objective To study the significance of the variations of serum neuron specific enolase (NSE) level in hypox-ic-ischemic encephalopathy (HIE) neonatorum. Methods The serum NSE level were detected in 75 asphyxia neonato-rum (asphyxia group) and 20 normal neonatomm (control group) by the method of ELISA after parturition 1,3 and 7 days. The relation of serum NSE level and hypoxic ischemic encephalopathy was analyzed. Results After parturition 1,3 and 7 days,the serum NSE level in asphyxia group was higher than that in control group [(44.50±12.08)μg/Lvs (15.59±6.25)μg/L,(42.65±12.43) μg/L vs (15.38±5.84) μg/L,(37.90±9.50) μg/L vs (13.92±5.37) μg/L],there had significant difference (P<0.05).The serum NSE level in sever asphyxia group was higher than that in mild asphyxia group [(58.95±14.85)μg/Lvs (29.15±9.32) μg/L, (62.95±19.13) μg/Lvs ( 22.35±5.75) μg/L, (57.35±13.75) μg/Lvs (18.45±5.25) μg/L],there had significant difference (P<0.05).After parturition 1,3 and 7 d,the serum NSE level in HIE neonatorum(19 cases) was in-creased and in MRI normal neonatorum (56 cases) was decreased,the serum NSE level in HIE neonatorum was higher than that in MRI normal neonatorum [(59.84±15.09) μg/Lvs (32.95±12.86)μg/L,(63.95±19.21)μg/Lvs (25.73±6.75)μg/L, (68.25±19.79) μg/L vs (19.62±5.94) μg/L],there had significant difference (P<0.05). Conclusion The variations of serum NSE level can be the important indicator to monitor HIE in asphyxia neonatorum.