CAJ | 학술논문

目的：探讨尼可地尔对阿霉素损伤大鼠心肌的保护作用。方法将45只雄性SD大鼠用随机数字表法分为对照组(A组，n=15)、阿霉素模型组(B组，n=15)、尼可地尔+阿霉素组(C组，n=15)。 B、C组大鼠每周经腹腔注射阿霉素2.5 mg/kg，A组大鼠每周经腹腔注射生理盐水2.5 mg/kg，共持续6周。第8周开始C组大鼠每次灌胃法灌入尼可地尔3 mg/kg，1次/d，持续30 d。之后通过心脏彩超检测三组大鼠的心脏功能并将其处死。用天狼星红染色法检测各组大鼠心肌的胶原容积分数。结果①造模结束时，B组大鼠平均体重小于A、C组(P<0.01或P<0.05)。②超声结果显示：与A组比较，B组大鼠左室收缩末内径(LVEDS)、收缩末期容积(ESV)明显增大(P<0.01)，左室射血分数(LVEF)、左室短轴收缩率(FS%)明显降低(P<0.01)；与B组比较，C组LVEDS、ESV明显减小(P<0.01)，LVEF、FS%明显增高(P<0.01)。③病理结果显示：B组心肌组织胶原容积分数明显比A组高(P<0.01)，而B、C两组比较差异无统计学意义(P>0.05)。结论尼可地尔对阿霉素损伤心肌有保护作用。
목적：탐토니가지이대아매소손상대서심기적보호작용。방법장45지웅성SD대서용수궤수자표법분위대조조(A조，n=15)、아매소모형조(B조，n=15)、니가지이+아매소조(C조，n=15)。 B、C조대서매주경복강주사아매소2.5 mg/kg，A조대서매주경복강주사생리염수2.5 mg/kg，공지속6주。제8주개시C조대서매차관위법관입니가지이3 mg/kg，1차/d，지속30 d。지후통과심장채초검측삼조대서적심장공능병장기처사。용천랑성홍염색법검측각조대서심기적효원용적분수。결과①조모결속시，B조대서평균체중소우A、C조(P<0.01혹P<0.05)。②초성결과현시：여A조비교，B조대서좌실수축말내경(LVEDS)、수축말기용적(ESV)명현증대(P<0.01)，좌실사혈분수(LVEF)、좌실단축수축솔(FS%)명현강저(P<0.01)；여B조비교，C조LVEDS、ESV명현감소(P<0.01)，LVEF、FS%명현증고(P<0.01)。③병리결과현시：B조심기조직효원용적분수명현비A조고(P<0.01)，이B、C량조비교차이무통계학의의(P>0.05)。결론니가지이대아매소손상심기유보호작용。
Objective To investigate the protection effect of Nicorandil on myocardial of rats dameged by Adriamycin. Methods 45 male SD rats were randomly divided into control group (group A, n=15), Adriamycin group (group B, n=15) and Adriamycin combined with Nicorandil group (group C, n=15). The rats in group B and C were performed with intraperitoneal injection of Adriamycin at the dose of 2.5 mg/kg a week , those in group A were performed with in-traperitoneal injection of normal saline at the dose of 2.5 mg/kg a week, for 6 weeks. From the eighth week, rats in group C were performed with Nicorandil by stomach lavaging at the dose of 3 mg/kg once a day for 30 days. Then the rats were sacrificed after cardiac function accessed by echocardiography. Meanwhile, collagen volume fraction was mea-sured in each group by sirius red staining. Results①At the end of molding, mean weight of rats in group B was lighter than those in group A and C (P< 0.01 or P< 0.05). ②Echocardiography showed that LVEDS and ESV in group B were obviously larger than those in group A and C (P<0.01), whlie LVEF and FS%were obviously lower than those in group A and C (P<0.01). ③The results of pathology showed that the collagen volume fraction in group B was higher than that in group A (P< 0.01), and there was no statistical difference between group B and group C in collagen vol-ume fraction (P>0.05). Conclusion Nicorandil has protection effects on myocardial of rats dameged by Adriamycin.