CAJ | 학술논문

目的：研究人工麝香对卵白蛋白(OVA)致敏引发的小鼠气道炎症损伤的保护作用及其机制。方法雄性Balb/c小鼠分别于第0天和第14天腹腔注射20μg OVA和2.25 mg Al(OH)3凝胶致敏，于第28、29、30天从气管滴入OVA进行攻击，攻击前1 h给予受试化合物人工麝香及阳性药地塞米松。小鼠随机分为6组院正常对照组(n=11)、模型组(OVA组，n=10)、地塞米松组(Dex组，n=10)、人工麝香给药10 d组(n=11)、人工麝香给药7 d组(n=13)、人工麝香给药5 d组(n=11)。人工麝香皮下给药100 mg/kg，分别自致敏第21、24、26天开始给药，给药周期为10、7、5 d。处死小鼠后，对小鼠的体重、脾脏和胸腺进行称重，计算脾脏、胸腺指数。检测支气管肺泡灌洗液(BALF)炎症细胞及炎症因子IL-4、IL-5及IL-17含量改变，HE染色分析小鼠肺部病理变化，免疫组化分析中性粒细胞生物标志物Ly6G/Gr-1的表达水平。结果与模型组相比，人工麝香100 mg/kg连续给药5、7 d可改善OVA致敏引发的小鼠气道炎症，明显降低小鼠BALF炎症细胞因子IL-4、IL-5、IL-17的含量(P<0.05)。细胞分类计数、肺组织病理分析及免疫组化结果表明，人工麝香能够抑制小鼠肺部气道中炎症细胞，特别是中性粒细胞的浸润，以及中性粒细胞标志物Ly6G/Gr-1的表达。结论人工麝香具有较强的减轻OVA致敏引发的小鼠气道炎症损伤的功能，提示人工麝香对于哮喘可能有一定的治疗作用。
목적：연구인공사향대란백단백(OVA)치민인발적소서기도염증손상적보호작용급기궤제。방법웅성Balb/c소서분별우제0천화제14천복강주사20μg OVA화2.25 mg Al(OH)3응효치민，우제28、29、30천종기관적입OVA진행공격，공격전1 h급여수시화합물인공사향급양성약지새미송。소서수궤분위6조원정상대조조(n=11)、모형조(OVA조，n=10)、지새미송조(Dex조，n=10)、인공사향급약10 d조(n=11)、인공사향급약7 d조(n=13)、인공사향급약5 d조(n=11)。인공사향피하급약100 mg/kg，분별자치민제21、24、26천개시급약，급약주기위10、7、5 d。처사소서후，대소서적체중、비장화흉선진행칭중，계산비장、흉선지수。검측지기관폐포관세액(BALF)염증세포급염증인자IL-4、IL-5급IL-17함량개변，HE염색분석소서폐부병리변화，면역조화분석중성립세포생물표지물Ly6G/Gr-1적표체수평。결과여모형조상비，인공사향100 mg/kg련속급약5、7 d가개선OVA치민인발적소서기도염증，명현강저소서BALF염증세포인자IL-4、IL-5、IL-17적함량(P<0.05)。세포분류계수、폐조직병리분석급면역조화결과표명，인공사향능구억제소서폐부기도중염증세포，특별시중성립세포적침윤，이급중성립세포표지물Ly6G/Gr-1적표체。결론인공사향구유교강적감경OVA치민인발적소서기도염증손상적공능，제시인공사향대우효천가능유일정적치료작용。
Objective To investigate the effects of artifitial musk on attenuation of OVA-challenged murine airway in-flammation in vivo and its mechanism. Methods Male Balb/c mice were divided into 6 groups, including control (n=11), OVA model (n=10), Dex (n=10), artifitial musk 10 days (n=11), artifitial musk 7 days (n=13), and artifitial musk 5 days (n=11). Mice were immunized intraperitoneally with 20 μg ovalbumin (OVA) and 4 mg Al (OH)3 suspended in 0.1 mL saline solution on Day 0 and Day 14, and then intratracheally challenged with 1% OVA suspended in saline solution on Days 28-30. Artifitial musk (100 mg/kg, s.c.) was given 1 hour before each OVA challenge for 5, 7 and 10 days since the sensitization for 21, 24, 26 days, respectively. After sacrificed, the body weights, spleen index and thymus in-dex were calculated. In addition, pathologic changes in the lung tissues, number of inflammatory cells in bronchoalveo-lar lavage fluid (BALF) and expression levels of IL-4, IL-5, IL-17 were observed. Moreover, the expression levels of Ly6G/Gr-1, a major biomarker of neutrophil, was detected by immunohistochemical examination. Results Compared with OVA model, the airway inflammation in mice improved after treatment with 100 mg/kg artifitial musk for 5 and 7 days in v iv o. Moreover, the expression levels of major cytokines as IL-4, IL-5 and IL-17 in BALF were inhibited after treatment with musk (P< 0.05). Pathological and cell count results showed that the inflammatory cell especially neu-trophile granulocyte infiltration in the airway section reduced after treatment with artifitial musk. Furthermore, immuno-histochemical examination results showed that the expression levels of Ly6G/Gr-1 were inhibited upon the treatment. Conclusion The results showed that artifitial musk pos-sesses inhibitory effects on airway inflammation induced with OVA in v iv o and the therapeutic function on asthma in the future.