中国当代医药
中國噹代醫藥
중국당대의약
PERSON
2014年
17期
15-17,20
,共4页
舒张性心力衰竭%L型钙通道%雷米普利%Cav1.2
舒張性心力衰竭%L型鈣通道%雷米普利%Cav1.2
서장성심력쇠갈%L형개통도%뢰미보리%Cav1.2
Diastolic heart failure%L-type calcium channel%Ramipril%Cav1.2
目的：观察雷米普利对舒张性心力衰竭(DHF)大鼠模型心房肌细胞L型钙通道的影响。方法腹主动脉缩窄术建立DHF大鼠模型，随机分为DHF组及雷米普利大、中、小剂量组，另设对照组。全细胞膜片钳检测心房肌细胞L型钙通道电流(ICa-L)；RT-PCR检测L型钙通道上Cav1.2 mRNA的表达；Western blot检测钙调控相关蛋白LTCC的表达。结果与对照组比较，DHF组大鼠心房肌细胞ICa-L电流密度峰值、Cav1.2 mRNA表达及钙调蛋白LTCC的表达明显降低(P<0.05)；与DHF组比较，雷米普利组心房肌细胞ICa-L电流密度峰值明显增加、Cav1.2 mRNA及钙调控蛋白LTCC的表达增加(P<0.05)，且剂量越大增加越明显。结论雷米普利能上调舒张性心力衰竭大鼠心房肌ICa-L电流密度峰值，增加Cav1.2 mRNA及钙调控蛋白LTCC的表达，对DHF大鼠有治疗作用，其机制与调控心房肌细胞L型钙通道的功能有关。
目的：觀察雷米普利對舒張性心力衰竭(DHF)大鼠模型心房肌細胞L型鈣通道的影響。方法腹主動脈縮窄術建立DHF大鼠模型，隨機分為DHF組及雷米普利大、中、小劑量組，另設對照組。全細胞膜片鉗檢測心房肌細胞L型鈣通道電流(ICa-L)；RT-PCR檢測L型鈣通道上Cav1.2 mRNA的錶達；Western blot檢測鈣調控相關蛋白LTCC的錶達。結果與對照組比較，DHF組大鼠心房肌細胞ICa-L電流密度峰值、Cav1.2 mRNA錶達及鈣調蛋白LTCC的錶達明顯降低(P<0.05)；與DHF組比較，雷米普利組心房肌細胞ICa-L電流密度峰值明顯增加、Cav1.2 mRNA及鈣調控蛋白LTCC的錶達增加(P<0.05)，且劑量越大增加越明顯。結論雷米普利能上調舒張性心力衰竭大鼠心房肌ICa-L電流密度峰值，增加Cav1.2 mRNA及鈣調控蛋白LTCC的錶達，對DHF大鼠有治療作用，其機製與調控心房肌細胞L型鈣通道的功能有關。
목적：관찰뢰미보리대서장성심력쇠갈(DHF)대서모형심방기세포L형개통도적영향。방법복주동맥축착술건립DHF대서모형，수궤분위DHF조급뢰미보리대、중、소제량조，령설대조조。전세포막편겸검측심방기세포L형개통도전류(ICa-L)；RT-PCR검측L형개통도상Cav1.2 mRNA적표체；Western blot검측개조공상관단백LTCC적표체。결과여대조조비교，DHF조대서심방기세포ICa-L전류밀도봉치、Cav1.2 mRNA표체급개조단백LTCC적표체명현강저(P<0.05)；여DHF조비교，뢰미보리조심방기세포ICa-L전류밀도봉치명현증가、Cav1.2 mRNA급개조공단백LTCC적표체증가(P<0.05)，차제량월대증가월명현。결론뢰미보리능상조서장성심력쇠갈대서심방기ICa-L전류밀도봉치，증가Cav1.2 mRNA급개조공단백LTCC적표체，대DHF대서유치료작용，기궤제여조공심방기세포L형개통도적공능유관。
Objective To observe the influence of ramipril on L-type calcium channel in rats with diastolic heart fail-ure (DHF). Methods The rat model of DHF was established by using abdominal aortic coarctation,and then the rats were randomly divided into DHF group,high-dose,mid-dose and low-dose ramipril groups and control group.L-type calcium channel current (ICa-L) was investigated by using a whole cell patchclamp technique.The expression levels of Cav1.2 mRNA were measured by RT-PCR analysis.The expression levels of LTCC were measured by Wsetern blot analysis. Results Compared with control group,the peak density of atrial ICa-L,the expression levels of Cav1.2 mRNA and the expression levels of LTCC was lower in DHF group (P<0.05).Compared with the DHF group,the peak density of atrial ICa-L,the expression levels of Cav1.2 mRNA and the expression levels of LTCC increased in three ramipril groups (P<0.05).The more dose of ramipril,the more increasing in ramipril group. Conclusion Ramipril can improve the peak density of atrial ICa-L,the expression levels of Cav1.2 mRNA and the expression levels of LTCC,has the thera-peutic effect on DHF in rats,and the mechanism may be related to improving the activties of L-type calcium channel.
