CAJ | 학술논문

目的：运用实时荧光定量PCR(qRT-PCR)技术检测miR-331-3p在肝癌细胞株和肝癌组织中的表达，探讨其在肝癌中表达的临床意义及潜在临床价值。方法采用基于2-△△Ct的qRT-PCR检测miR-331-3p在正常肝细胞株(HL-7702)、不同侵袭转移能力的肝癌细胞株(HepG2、MHCC97-H、HCCLM3)的表达，同时收集5例正常肝组织、30例肝癌组织及癌旁组织，进行定量分析，并分析miR-331-3p与肝癌患者临床病理特征的关系。结果与正常肝细胞株相比，miR-331-3p在肝癌细胞株中表达下调，且随着肝癌细胞株侵袭转移程度增加，其表达下调越明显(P<0.05)；与正常肝组织相比，肝癌组织中miR-331-3p有不同程度的表达下调(P<0.05)，且与肝癌患者肿瘤多发结节(P=0.036)、低分化程度(P=0.035)以及伴有静脉浸润(P=0.016)等临床病理特征相关。结论 miR-331-3p在肝癌的发生、发展过程可能发挥重要作用，miR-331-3p有望成为肝癌新的生物标志物或预后因子。
목적：운용실시형광정량PCR(qRT-PCR)기술검측miR-331-3p재간암세포주화간암조직중적표체，탐토기재간암중표체적림상의의급잠재림상개치。방법채용기우2-△△Ct적qRT-PCR검측miR-331-3p재정상간세포주(HL-7702)、불동침습전이능력적간암세포주(HepG2、MHCC97-H、HCCLM3)적표체，동시수집5례정상간조직、30례간암조직급암방조직，진행정량분석，병분석miR-331-3p여간암환자림상병리특정적관계。결과여정상간세포주상비，miR-331-3p재간암세포주중표체하조，차수착간암세포주침습전이정도증가，기표체하조월명현(P<0.05)；여정상간조직상비，간암조직중miR-331-3p유불동정도적표체하조(P<0.05)，차여간암환자종류다발결절(P=0.036)、저분화정도(P=0.035)이급반유정맥침윤(P=0.016)등림상병리특정상관。결론 miR-331-3p재간암적발생、발전과정가능발휘중요작용，miR-331-3p유망성위간암신적생물표지물혹예후인자。
Objective To detect the expression of miR-331-3p in hepatocellular carcinoma (HCC) by quantificational real-time polymerase chain reaction (qRT-PCR) and investigate the significance and potential clinical value of miR-331-3p in HCC. Methods 2-△△Ct method was used for qRT-PCR of the expression pattern of miR-331-3p in normal human liv-er cell line HL-7702 and different invasion and metastasis of HCC cell lines (HepG2,MHCC97-H,HCCLM3),mean-while,5 normal liver tissues,30 HCC and adjacent tissues were selected,and the relationship between the expression of miR-331-3p and the clinicopathological characteristics of HCC was analyzed. Results Compared with normal liver cell line,hepatocellular carcinoma cell lines showed miR-331-3p down-regulation,and with the increase of the degree of invasion and metastasis,the more obvious its down-regulation (P<0.05).Compared with normal liver tissues,the HCC tis sues showed significant miR-331-3p down-regulation (P<0.05),and it was related to multiple tumor nodules (P=0.036),low differentiation (P=0.035),and venous invasion (P=0.016). Conclusion In the process of the occurrence and development of HCC,miR-331-3p may play an important role,it is expected to become the new biomarker or prognostic factor of HCC.