CAJ | 학술논문

目的：研究肿瘤坏死因子相关凋亡诱导配体（TRAIL）处理前列腺癌细胞能否诱导自噬以及自噬在其中的作用。方法利用免疫印迹技术检测自噬相关蛋白的表达；通过抑制自噬观察 TRAIL对前列腺癌细胞毒性的影响。结果 TRAIL可以增强自噬相关蛋白的表达，抑制自噬可以增强TRAIL对前列腺癌细胞的毒性作用。结论 TRAIL可以诱导自噬的发生，自噬对前列腺癌细胞起保护作用。
목적：연구종류배사인자상관조망유도배체（TRAIL）처리전렬선암세포능부유도자서이급자서재기중적작용。방법이용면역인적기술검측자서상관단백적표체；통과억제자서관찰 TRAIL대전렬선암세포독성적영향。결과 TRAIL가이증강자서상관단백적표체，억제자서가이증강TRAIL대전렬선암세포적독성작용。결론 TRAIL가이유도자서적발생，자서대전렬선암세포기보호작용。
Objective To study whether the TRAIL treating prostate cancer cells can induce autophagy and the role of autoph-agy in it.Methods The expression of autophagy-related protein was detected by Western blot;the toxicity of TRAIL on prostate cancer cells was observed by suppressing autophagy.Results TRAIL could enhance the expression of autophagy-related protein,in-hibiting autophagy could enhance the cytotoxic effect of TRAIL on prostate cancer cells.Conclusion TRAIL can induce the autoph-agy occurrence in prostate cancer and autophagy play a protective role on prostate cancer cells.