CAJ | 학술논문

目的：研究电针对非酒精性脂肪肝（NAFLD）大鼠肝细胞角蛋白18（CK18）表达的影响。方法将 Wistar大鼠分为正常组和造模组，造模组大鼠模型建立成功后，再分为 NAFLD模型组、药物组、电针组，药物组给予东宝肝泰灌胃，电针组针刺脾俞、丰隆、阳陵泉、三阴交穴。治疗4周后处死所有动物，观察肝组织病理学变化情况；采用比色法检测肝内丙二醛（MDA）含量、超氧化物歧化酶（SOD）活性的变化，免疫组化法检测CK18的表达。结果与 NAFLD模型组比较，电针组和药物组大鼠肝组织脂肪变性得到不同程度的减轻；肝脏CK18表达均受抑制；肝内 MDA均有不同程度的降低（P＜0．01；P＜0．05），SOD活性升高（P＜0．01；P＜0．05）。结论电针治疗可能通过抑制肝脏CK18表达的上调，减轻脂质过氧化反应。
목적：연구전침대비주정성지방간（NAFLD）대서간세포각단백18（CK18）표체적영향。방법장 Wistar대서분위정상조화조모조，조모조대서모형건립성공후，재분위 NAFLD모형조、약물조、전침조，약물조급여동보간태관위，전침조침자비유、봉륭、양릉천、삼음교혈。치료4주후처사소유동물，관찰간조직병이학변화정황；채용비색법검측간내병이철（MDA）함량、초양화물기화매（SOD）활성적변화，면역조화법검측CK18적표체。결과여 NAFLD모형조비교，전침조화약물조대서간조직지방변성득도불동정도적감경；간장CK18표체균수억제；간내 MDA균유불동정도적강저（P＜0．01；P＜0．05），SOD활성승고（P＜0．01；P＜0．05）。결론전침치료가능통과억제간장CK18표체적상조，감경지질과양화반응。
Objective To investigate the influence of electroacupuncture(EA)intervention on liver CK18 expression in nonalco-holic fatty liver (NAFL)rats.Methods Wistar rats were randomly divided into the normal group and the model group.After suc-cessfully establishing the rat model,the model group was redivided into the NAFLD group,drug group and EA group.The drug group was fed with Dongbao Gantai by gavage and the EA group was performed the EA acupuncture at the specific points of unilat-eralPishu,Fenglong,YanglingquanandSanyinj iao.After 4-week treatment,all animals were killed and the live histopatho-logical changes were observed.The contents of liver malondialdehyde (MDA)and superoxide dismutase (SOD)were detected by the colorimetric method.The liver CK18 expression was detected by the mmunohistochemical method.Results Compared with the model group,the steatosis of the liver tissue in the EA group and the drug group were alleviated to different extents;expression of hepatic CK18 was suppressed;liver MDA was decreased with various degrees (P<0.01;P<0.05)and the SOD activity was in-creased (P<0.01;P<0.05).Conclusion Electroacupuncture may reduce lipid peroxidation by inhibiting the up-regulation of he-patic CK18 expression.