CAJ | 학술논문

目的：探讨美洲大蠊提取物（APA）对实验性肝纤维化组织中核转录因子-κB（NF-κB）和α-平滑肌肌动蛋白（α-SMA）表达的影响，并观察 APA的抗纤维化疗效。方法采用四氯化碳复制大鼠肝纤维化模型，同时用0．5 g/kg的 APA灌胃治疗，测定血清中丙氨酸氨基转移酶（ALT）、天门冬氨酸氨基转移酶（AST）、清蛋白（Alb）水平。免疫组化测定肝组织中NF-κB和α-SMA表达的水平，并以光镜观察肝病理组织学改变，进行炎性活动度分级及纤维化分期。结果 APA可以降低血清中 ALT、AST和肝组织中 NF-κB、α-SMA的水平，APA组和三七丹参组与模型组相比血清中 Alb水平差异有统计学意义，用药后的肝脏细胞变性坏死及纤维化程度明显降低。结论 APA具有一定的抗纤维化作用，机制可能与其抑制肝组织中 NF-κB和α-SMA的表达有关。
목적：탐토미주대렴제취물（APA）대실험성간섬유화조직중핵전록인자-κB（NF-κB）화α-평활기기동단백（α-SMA）표체적영향，병관찰 APA적항섬유화료효。방법채용사록화탄복제대서간섬유화모형，동시용0．5 g/kg적 APA관위치료，측정혈청중병안산안기전이매（ALT）、천문동안산안기전이매（AST）、청단백（Alb）수평。면역조화측정간조직중NF-κB화α-SMA표체적수평，병이광경관찰간병리조직학개변，진행염성활동도분급급섬유화분기。결과 APA가이강저혈청중 ALT、AST화간조직중 NF-κB、α-SMA적수평，APA조화삼칠단삼조여모형조상비혈청중 Alb수평차이유통계학의의，용약후적간장세포변성배사급섬유화정도명현강저。결론 APA구유일정적항섬유화작용，궤제가능여기억제간조직중 NF-κB화α-SMA적표체유관。
Objective To investigate the effect of American cockroach extract on the expression of NF-κB andα-SMA in experi-mental liver fibrosis tissue,and to observe its antifibrotic efficacy.Methods The rat model of hepatic fibrosis was duplicated by car-bon tetrachloride and simultaneously given with American cockoach extract 0.5 g/kg by lavage.The level of serum alanine amin-otransferase enzyme (ALT),aspartate aminotransferase (AST)and albumin (Alb)were determined.The expression levels of NF-κB and a-SMA in liver tissue were determined by the immunohistochemical method and the liver histological changes were observed by light microscopy for conducting the grading of inflammatory activity and the fibrosis classification.Results American cockroach extract could reduce serum ALT,AST and liver tissue NF-κB andα-SMA levels.The serum Alb level had significant difference a-mong the APA group,Pseudo-ginseng Danshen group and the model group.The degeneration and necrosis of liver cells and the fi-brosis degree after medication were significantly reduced.Conclusion The American cockroach extract has certain anti-fibrosis effect,its mechanism may be related with the inhibition of expression of NF-κB andα-SMA.