重庆医学
重慶醫學
중경의학
CHONGQING MEDICAL JOURNAL
2014年
17期
2149-2151,2154
,共4页
朱晋坤%毛华%尹扬光%董文%邓梦扬%鲁玉明%熊宗华%杜峰%文美%刘廷筑
硃晉坤%毛華%尹颺光%董文%鄧夢颺%魯玉明%熊宗華%杜峰%文美%劉廷築
주진곤%모화%윤양광%동문%산몽양%로옥명%웅종화%두봉%문미%류정축
血小板衍化内皮细胞生长因子%冠状动脉再狭窄%经皮冠状动脉介入治疗
血小闆衍化內皮細胞生長因子%冠狀動脈再狹窄%經皮冠狀動脈介入治療
혈소판연화내피세포생장인자%관상동맥재협착%경피관상동맥개입치료
platelet-derived-endothelial-cell-growth-factor%coronary-restenosis%percutaneous-coronary-intervention
目的：通过在兔颈动脉球囊损伤模型中转染血小板衍化内皮细胞生长因子（PD-ECGF）基因使其上调，探讨该基因在预防血管再狭窄中的作用。方法36只新西兰白兔（体质量2．5～3．0 kg）建立兔颈动脉球囊损伤模型，分为实验组（n＝18）和对照组（n＝18）：实验组使用病毒进行 PD-ECGF基因转染；对照组采用交付剂处理。3周后，采用 Evans蓝染色和 HE染色分别检测各组内皮覆盖率及新生内膜的改变情况；采用免疫组化技术和免疫印迹法检测颈动脉组织中 PD-ECGF蛋白表达情况。结果实验组内皮覆盖率[（72．8±4．0）％]显著高于对照组[（33．6±7．4）％]；HE染色可见实验组内膜及中层平滑肌增生程度较对照组明显减轻；免疫组化及免疫印迹法结果均表明实验组颈动脉组织中 PD-ECGF蛋白表达显著高于对照组（P＜0．05）。结论在家兔颈动脉球囊损伤模型中，上调PD-ECGF基因具有抑制平滑肌细胞增殖和促进内皮细胞生长以及血管生成的作用。
目的：通過在兔頸動脈毬囊損傷模型中轉染血小闆衍化內皮細胞生長因子（PD-ECGF）基因使其上調，探討該基因在預防血管再狹窄中的作用。方法36隻新西蘭白兔（體質量2．5～3．0 kg）建立兔頸動脈毬囊損傷模型，分為實驗組（n＝18）和對照組（n＝18）：實驗組使用病毒進行 PD-ECGF基因轉染；對照組採用交付劑處理。3週後，採用 Evans藍染色和 HE染色分彆檢測各組內皮覆蓋率及新生內膜的改變情況；採用免疫組化技術和免疫印跡法檢測頸動脈組織中 PD-ECGF蛋白錶達情況。結果實驗組內皮覆蓋率[（72．8±4．0）％]顯著高于對照組[（33．6±7．4）％]；HE染色可見實驗組內膜及中層平滑肌增生程度較對照組明顯減輕；免疫組化及免疫印跡法結果均錶明實驗組頸動脈組織中 PD-ECGF蛋白錶達顯著高于對照組（P＜0．05）。結論在傢兔頸動脈毬囊損傷模型中，上調PD-ECGF基因具有抑製平滑肌細胞增殖和促進內皮細胞生長以及血管生成的作用。
목적：통과재토경동맥구낭손상모형중전염혈소판연화내피세포생장인자（PD-ECGF）기인사기상조，탐토해기인재예방혈관재협착중적작용。방법36지신서란백토（체질량2．5～3．0 kg）건립토경동맥구낭손상모형，분위실험조（n＝18）화대조조（n＝18）：실험조사용병독진행 PD-ECGF기인전염；대조조채용교부제처리。3주후，채용 Evans람염색화 HE염색분별검측각조내피복개솔급신생내막적개변정황；채용면역조화기술화면역인적법검측경동맥조직중 PD-ECGF단백표체정황。결과실험조내피복개솔[（72．8±4．0）％]현저고우대조조[（33．6±7．4）％]；HE염색가견실험조내막급중층평활기증생정도교대조조명현감경；면역조화급면역인적법결과균표명실험조경동맥조직중 PD-ECGF단백표체현저고우대조조（P＜0．05）。결론재가토경동맥구낭손상모형중，상조PD-ECGF기인구유억제평활기세포증식화촉진내피세포생장이급혈관생성적작용。
Objective To investigate the role of PD-ECGF gene in preventing the vascular restenosis by up-regulating the PD-ECGF gene in the rabbits′carotid artery balloon injury model.Methods 36 New Zealand white rabbits (body weight 2.5-3.0 kg) were established the rabbits′carotid artery balloon injury model and randomly divided into the experimental group (n=18)and the control group(n=18).The experimental group was received the PD-ECGF gene transfection by virus and the control group was treated with the delivery agent.After 3 weeks,the intima coverage rate was tested by Evans blue staining.HE staining was used to investigate the morphological alteration of neointima and accurately measure the area of arterial intima.Finally,the expression of PD-ECGF protein was determined by the immunohistochemical technique and Western blot.Results The intima coverage rate in the experimental group was significantly higher than that in the control group (72.8±4.0%vs.33.6±7.4%,P<0.05);the HE staining showed that the hyperplasia degrees of intima and middle-lever smooth muscle in the experimental group were significantly alleviated than the control group;moreover,the immunohistochemical and Western blot results showed that the expression of PD-ECGF protein in the experimental group was significantly higher than that in the control group (P<0.05).Conclusion Up-regula-ting PD-ECGF gene has the role for inhibiting the proliferation of vascular smooth muscle cells and promoting the epithelial cell growth and angiogenesis in rabbits′carotid artery balloon inj ury model.
