CAJ | 학술논문

目的：探讨小青龙汤对卵蛋白致敏/激发小鼠气道变应性炎症的作用。方法 BALB/c小鼠以 OVA致敏/激发，在其每日供水时加入小青龙汤；在最后一次激发24 h后留取标本，检测肺泡灌洗液（BALF）中细胞计数和分类，测定支气管 BALF、血清中细胞因子以及外周血和BALF中 Foxp3＋CD4＋ T细胞的比例。结果哮喘组可见明显炎性细胞浸润，经小青龙汤干预后肺部炎性细胞浸润显著减少；哮喘组 BALF中炎性细胞，特别是嗜酸性粒细胞计数较对照组明显升高（P＜0．01）；白细胞介素（IL）-4、IL-5水平较对照组明显升高（P＜0．01），Foxp3＋T细胞比例显著下降（P＜0．01），Th1细胞因子干扰素（IFN）-γ无显著变化，治疗组与哮喘组比较，IL-4、IL-5水平、Foxp3＋T细胞比例增加，差异无统计学意义（P＞0．05）；IL-10水平显著升高。结论小青龙汤可有效抑制哮喘模型小鼠气道变应性炎症，其作用途径可能是通过诱导 Foxp3＋调节性T细胞产生、IL-10的分泌，进而抑制了Th2型免疫反应。
목적：탐토소청룡탕대란단백치민/격발소서기도변응성염증적작용。방법 BALB/c소서이 OVA치민/격발，재기매일공수시가입소청룡탕；재최후일차격발24 h후류취표본，검측폐포관세액（BALF）중세포계수화분류，측정지기관 BALF、혈청중세포인자이급외주혈화BALF중 Foxp3＋CD4＋ T세포적비례。결과효천조가견명현염성세포침윤，경소청룡탕간예후폐부염성세포침윤현저감소；효천조 BALF중염성세포，특별시기산성립세포계수교대조조명현승고（P＜0．01）；백세포개소（IL）-4、IL-5수평교대조조명현승고（P＜0．01），Foxp3＋T세포비례현저하강（P＜0．01），Th1세포인자간우소（IFN）-γ무현저변화，치료조여효천조비교，IL-4、IL-5수평、Foxp3＋T세포비례증가，차이무통계학의의（P＞0．05）；IL-10수평현저승고。결론소청룡탕가유효억제효천모형소서기도변응성염증，기작용도경가능시통과유도 Foxp3＋조절성T세포산생、IL-10적분비，진이억제료Th2형면역반응。
Objective To investigate the effects of Chinese herbal prescription Xiaoqinglongtang (XQLT)on the airway allergic inflammation BALB/c mice model sensitized and challenged with ovalumin (OVA).Methods BALB/c mice were sensitized and challenged with OVA.XQLT was added in the daily water supply.After 24 h of last challenge,the sample was taken for detecting the cells count and classification in the bronchoalveolar lavage fluid(BALF),cytokins in BALF and serum and the ratio of Foxp3+CD4+ T cells in peripheral blood and BALF.Results The obvious inflammatory cell infiltration could be seen in the asthma mice. After XQLT intervention,the pulmonary inflammatory cell infiltration was significantly decreased;the inflammatory cells in BALF in the asthma mice,especially eosinophilic granulocytes count was significantly increased compared with the control group (P<0.01);the IL-4 and IL-5 levels were significantly elevated compared with the control group(P<0.01),the ratio of Foxp3+T cells was significantly decreased(P<0.01).Th1 cytokine IFN-γhad no obvious change.The cell count,IL-4 and IL-5 levels,and the ra-tio of Foxp3+T cells in the XQLT intervention group were significantly increased compared with the asthma group,but which had no statistical differences compared with the control group;the IL-10 level was significantly increased.Conclusion XQLT could ef-ficiently inhibit the allergic airway inflammation in the asthma model mice,its role pathway may be inducing the generation of Foxp3+ regulatory T cells,IL-10 secreation,and then inhibit the Th2 type immune reaction.